-
Pump
-> Steel filter tank
-> [A/E filter ->
Durapore] in Stainless
Steel or Polycarbonate
Manifold -> DI rinsed
cubie container (the one
the sample was
in)
-
Clamp
all tubes to manifolds
and filter tanks
-
Make
sure bleeder valves are
on top
-
Turn
on pump and then bleed
A/E then Durapore and
the A/E again (turn
manifold on side,
bleeder valves
should be on highest
side of manifold)
-
Keep
cubie container on ice-
water will be used for
viral concentration
-
When
finished filtering:
(KEEP PUMP ON UNTIL ALL
FILTERS HAVE BEEN
REMOVED)
-
Place
“out” tube from
Durapore onto a filter
flask that is attached
to the vacuum side of
the pump (make sure
white trap is on tube so
no seawater can get into
the pump.
-
Wait
until water stops moving
towards the vacuum and
only bubbles are coming
through (if opened to
fast
bubbles go backwards)
-
Open
steel filter tank lid
and slowly open tank
bleeder valve to
depressurize (SLOWLY)
-
When
pressure has dropped
significantly push in
steel tank lid, allow a
few more minutes for
water to drain
through
-
Disconnect
clamp from bottom side
of A/E filter, remove
tube from “out” side
of A/E
-
Place
Durapore on knees and
swirl gently so all
water goes into the
filter flask
-
Once
water is out, open
manifold slowly, fold up
filter and place in
whirlpack. Should see
nothing on it.
-
Remove
Durapore “out” tube
from filter flask, place
A/E “out” tube onto
filter flask (need to
reattach “in”
Durapore to “out”
A/E.)
-
Place
A/E on knees, let water
drain out into the
filter flask as before
-
Open
manifold slowly, peel
off ring of filter,
discard, fold filter and
place in whirlpack. Will
be tinted in
color
-
Okay
to turn off pump now
-
If
pump is turned off
before filtering is over
you need to close steel
tank lines so the whole
system stays pressurized.
Use the black levers or
knobs on the steel tanks
to do this.
