Metabolic Regulation Laboratory is a combination of three labs whose overall
research interests are to determine the factors that regulate carbohydrate,
lipid metabolism, and protein in liver and skeletal muscle.
- To determine the intramuscular
and hormonal factors that regulate the utilization of fatty acids by skeletal
- To determine the mechanisms by
which long-chain fatty acids traverse the plasma membrane of muscle cells.
- To determine the level of impairment
in the regulation of fatty acid metabolism in muscle cell in the presence
of obesity and insulin resistance.
- To determine the impact of chronic
physical activity upon hepatic glucose and glycogen metabolism.
- To elucidate the mechanism by
which portal vein glucosensors detect changes in blood glucose concentration
and mediate the appropriate metabolic responses.
The Metabolic Regulation Lab is located
in Stauffer Hall of Sciences, rooms 166, 168, 169, 170 and in the Physical Education
Building, room B13.
- Experimental models used in
the lab include: Hindlimb perfusion system, in situ liver perfusion,
isolated cells incubation, cell culture, in site muscle incubation, and whole
body turnover studies.
- Analytical techniques used
in the lab include: Measurement of enzyme activity and metabolite levels
by spectrophotometry, fluorometry and radio-immunoassay; hormone assays via
radio-immunoassay and radioenzymatic assay; separation of membrane fractions
using ultracentrifugation; isolation and separation of metabolites using ion
exchange and thin layer chromatography; quantification of protein levels using
gel electrophoresis and immunoblotting; measurement of metabolite turnover
using tracer methodology.
- Equipment: Packard liquid
scintillation counter, Packard gamma counter, Beckman ultracentrifuge, Jouan
high speed centrifuge, general preparative centrifuges, microfuges, Hitachi
UV/Vis spectrophotometers, fluorometer, perfusion chambers, ultra low freezers,
cryostorage units, Radiometer Acid-Base Laboratory, and multiple YSI glucose
and lactate analyzers.
Current Research Projects
- Regulation of fatty acid metabolism
in muscle. Elucidation of the mechanisms by which carbohydrates interact with
fatty acids in oxidative metabolism.
- Effects of aging on fatty acid
metabolism in muscle. Elucidation of the mechanisms by which oxidative metabolism
is modified during aging.
- Regulation of fatty acid uptake
in muscle. Elucidation of the mechanisms by which fatty acids traverse the
plasma membrane of muscle cells.
- The impact of endurance training
upon the sensitivity and responsiveness of glucagon stimulated hepatic gluconeogenesis.
- Elucidation of the portal vein
glucosensor in normal and pathophysiological states.
- 1998-2003, NIH AR45168, "Exercise
and skeletal muscle fatty acid metabolism"
- 2001-2002, ACSM, "Effects
of insulin and glycolytic flux on muscle metabolism"
- 2001-2002, ACSM, "Effects
of training on lipid transport mechanisms in muscle"
- 2000-2002, Women in Science and
Engineering Supplemental Research grant, "FA metabolism and ultracellular
signaling in cultured muscle cells"
- 2000-2001, ACSM, "Effects
of thiazolidinediones on substrate utilization in muscle"
- 1996-1999, NIH DK48000, "Exercise
Adapations in Hepatic Gluconeogenesis"
- 1998-2001, NIH DK55257, "Portal
Glucosensors in Hypoglycemic Detection"