Protocol Online is a database of research protocols in a variety of life science fields. It contains protocols contributed by worldwide researchers as well as links to web protocols hosted by worldwide research labs, biotech companies, personal web sites. The data is stored in a MySql relational database. Protocol Online also hosts discipline specific discussion forums and mailing lists, and provides a free PubMed search and alerting service. This site, created in June 1999, is maintained by Dr. Long-Cheng Li. In order to better serve the research community, any comments and suggestions that can help improve this site are welcomed.