CCMB Faculty

CSA A-207, HSC 9062
(323)442-2556 Voice
(323)442-2981 Fax
anhle@usc.edu

Anh D. Le

D.D.S., Ph.D.

Associate Professor

Education Experience Honors & Awards Projects Publications

Education

INSTITUTION AND LOCATION	DEGREE	YEAR(s)	FIELD OF STUDY
University of California, Los Angeles	Certificate	1998	Oral/Maxillofacial Surgery
University of California, Los Angeles	Ph.D.	1998	Biology
University of California, Los Angeles	D.D.S.	1992	Dentistry
University of California, San Diego		1984-1985	Chemistry
University of California, San Diego	B.A.	1982	Biochem./Cell Biology

Research & Professional Experience

2006-	Member, Committee on Research, Oral and Maxillofacial Surgery Research Foundation
2006-	Advisory Committee on Research Planning and Technology Assessment, American Association of Oral and Maxillofacial Surgeons
2006	Reviewer, Study Session on Stem Cell Research, Joint Program of the Swedish Research Council (SRC), the Swedish Diabetes Association Research Foundation (SDARF), and the Juvenile Diabetes Research Foundation (JDRF)
2006	Reviewer, External Board Committee, Berzelius Center for Stem Cells, Swedish Research Council
2005-present	Associate Professor, School of Dentistry, University of Southern California
2004-present	Oral/Maxillofacial Surgery Attending, Los Angeles County-USC Medical Center
2004-2005	Visiting Professor, School of Dentistry, University of Southern California
2005	Peer reviewer committee, Small Proposals for the Jonsson Alper Program of Environmental Genomics, Molecular Epidemiology, UCLA Cancer Center
2005	Ad hoc reviewer, Cancer Research, Clinical Cancer Research, Molecular Cancer Therapeutics, Journal of Investigative Dermatology, International J. Oral & Maxillofacial Surgery, British J. Oral & Maxillofacial Surgery
2004	Ad hoc reviewer, Human Genetics, Cellular Immunology, Clinical Orthodontics and Research
2003	Associate Professor, Charles Drew University of Medicine and Science
2003	Ad hoc reviewer, Cellular Immunology
2003	Ad hoc reviewer, March of Dimes
2003	Diplomate, American Board of Oral and Maxillofacial Surgery
2002-2003	Assistant Professor, In-Residence, University of California, Los Angeles, School of Dentistry
2001-	Ad hoc reviewer, Clinical Orthodontics and Research
2001-	Ad hoc reviewer, J. National Medical Association
1999-2003	Assistant Professor, Charles Drew University of Medicine and Science
1999-2004	Oral/Maxillofacial Surgery Attending, King/Drew Medical Center
1998	Research Assistant Dentist, UCLA School of Dentistry
1984-1985	Teaching Assistant, University of California, San Diego, Department of Chemistry
1984-1985	Staff Research Associate, Department of Infectious Disease, San Diego Veteran Administration Hospital
1982-1984	Staff Research Associate, University of California, San Diego, Cancer Center

Academic Honors and Awards

2004-	Fellow, American Association of Oral and Maxillofacial Surgeons
2004-	Fellow, California Association of Oral and Maxillofacial Surgery
2004	President, Oral and Maxillofacial Surgery Group, IADR/AADR
2003	Vice-President, Oral and Maxillofacial Surgery Group, IADR/AADR
2001-2002	Oral and Maxillofacial Surgery Foundation Fellowship, Mentor
2001-2002	Surgical Attending of the Year, Oral and Maxillofacial Surgery Residency Training, King Drew Medical Center
2001	Group Program Chair, Oral and Maxillofacial Surgery Group, IADR/AADR
2000-2001	Oral and Maxillofacial Surgery Foundation Fellowship, Mentor
1996-2001	K08 DE00389 Award, “Regulation of Wound Angiogenesis: VEGF/VEGF Receptors”, NIDCR/NIH
1996	Woman of the Year, Dentistry
1994-1995	Oral and Maxillofacial Surgery Foundation Fellowship, Recipient
1994	Dean’s Fellowship
1992	Omikron Kappa Upsilon, National Dental Honorary
1984-1985	California State Fellowship

Research Projects

"Hypoxia regulation of VEGF/VEGF receptors in Keloids"

Principal Investigator: Anh D. Le, D.D.S., Ph.D.
Agency: National Institute of Arthritis and Musculoskeletal and Skin Diseases
Type: S11 AR47359, Period: Sep. 30, 2000 to Aug. 31, 2006
The major goal is this project is to study mechanism of hypoxia induced up-regulation of VEGF and its receptors in keloid dermal fibroblasts and microvascular endothelial cells and how these pathways contribute to ECM accumulation.

"Chemoprevention of Oral Cancer with BBIC"

Site Principal Investigator: Anh D. Le, D.D.S., Ph.D.
Agency: National Cancer Institute
Type: U01 CA 072294, Period: Sep. 1, 2003 to Aug. 31, 2008
The major goal of this phase IIb trial is to determine the effect of oral BBIC, a purified soybean extract, in oral cancer prevention.

"Scarless Wound Repair"

Site Principal Investigator: Anh D. Le, D.D.S., Ph.D.
Agency: National Institute of Arthritis and Musculoskeletal and Skin Diseases
Type: R43 AR051620, Period: Feb. 1, 2005 to Aug. 31, 2005
The major goal of this project is to study the effect of fibromodulin, a TGF beta modulator, on the healing and inflammation of incisional and excisional wounds in an animal model.

"Regulation of Wound Angiogenesis: VEGF and VEGF Receptors"

Principal Investigator: Anh D. Le, D.D.S., Ph.D.
Agency : National Institute of Dental and Craniofacial Research
Type: K08 DE00389, Period: 1996 to 2001
The major goal of this project is to study the expression of VEGF and its receptors by dermal microvascular endothelial cells and their biological functions in keloid pathogenesis.

"Matrix Catabolism in Abnormal Scarring CD44 and Hyaluronan"

Co-Principal Investigator: Anh D. Le, D.D.S., Ph.D.
Agency: National Institute of Dental and Craniofacial Research"
Type: P50 DE10598, Period: 1995 to 2000
The major goal of this project is to study the role of CD44 and its ligand, hyaluronan, in ECM accumulation in keloid fibrosis.

Publications

Zhang Q, Tang X, Zhang ZF, Velikina R, Shi S, Le AD. (2007) Nicotine induces hypoxia-inducible factor-1alpha expression in human lung cancer cells via nicotinic acetylcholine receptor-mediated signaling pathways. Clin Cancer Res. 13(16):4686-94.

Tang X, Zhang Q, Nishitani J, Brown J, Shi S, Le AD. (2007). Overexpression of human papillomavirus type 16 oncoproteins enhances hypoxia-inducible factor 1 alpha protein accumulation and vascular endothelial growth factor expression in human cervical carcinoma cells. Clin Cancer Res. 13(9):2568-76.

Lu QY, Jin YS, Zhang ZF, Le AD, Heber D, Li FP, Dubinett SM, Rao JY. (2007). Green tea induces annexin-I expression in human lung adenocarcinoma A549 cells: involvement of annexin-I in actin remodeling. Lab Invest. 87(5):456-65.

Zhang Q, Kelly AP, Wang L, French SW, Tang X, Duong HS, Messadi DV, Le AD. (2006). Green Tea Extract and (-)-Epigallocatechin-3-Gallate Inhibit Mast Cell-Stimulated Type I Collagen Expression in Keloid Fibroblasts via Blocking PI-3K/Akt Signaling Pathways. J Invest Dermatol. 126(12):2607-13

Zhang Q, Tang X, Lu Q, Zhang Z, Rao J, Le AD. (2006). Green tea extract and (-)-epigallocatechin-3-gallate inhibit hypoxia- and serum-induced HIF-1alpha protein accumulation and VEGF expression in human cervical carcinoma and hepatoma cells. Mol Cancer Ther. 5(5):1227-38.

Cai L, You NC, Lu H, Mu LN, Lu QY, Yu SZ, Le AD, Marshall J, Heber D, Zhang ZF. (2006). Dietary selenium intake, aldehyde dehydrogenase-2 and X-ray repair cross-complementing 1 genetic polymorphisms, and the risk of esophageal squamous cell carcinoma. Cancer. 106(11):2345-54

Cai L, Mu LN, Lu H, Lu QY, You NC, Yu SZ, Le AD, Zhao J, Zhou XF, Marshall J, Heber D, Zhang ZF. (2006). Dietary selenium intake and genetic polymorphisms of the GSTP1 and p53 genes on the risk of esophageal squamous cell carcinoma. Cancer Epidemiol Biomarkers Prev. 15(2):294-300.

Duong HS, Zhang QZ, Le AD, Kelly AP, Kamdar R, Messadi DV. (2006). Elevated prolidase activity in keloids: correlation with type I collagen turnover. Br J Dermatol. 154(5):820-8.

Zhang Q, Oh CK, Messadi DV, Duong HS, Kelly AP, Soo C, Wang L, Le AD. (2006). Hypoxia-induced HIF-1 alpha accumulation is augmented in a co-culture of keloid fibroblasts and human mast cells: involvement of ERK1/2 and PI-3K/Akt. Exp Cell Res. 312(2):145-55.

Chuang SC, Hashibe M, Yu GP, Le AD, Cao W, Hurwitz EL, Rao JY, Neugut AI, Zhang ZF. (2006). Radiotherapy for primary thyroid cancer as a risk factor for second primary cancers. Cancer Lett. 238(1):42-52

Duong HS, Le AD, Zhang Q, Messadi DV. (2005). A novel 3-dimensional culture system as an in vitro model for studying oral cancer cell invasion. Int J Exp Pathol. 86(6):365-74.

Zhang Q, Tang X, Lu QY, Zhang ZF, Brown J, Le AD. (2005). Resveratrol inhibits hypoxia-induced accumulation of hypoxia-inducible factor-1alpha and VEGF expression in human tongue squamous cell carcinoma and hepatoma cells. Mol Cancer Ther. 4(10):1465-74.

Hashibe M, Ritz B, Le AD, Li G, Sankaranarayanan R, Zhang ZF. (2005). Radiotherapy for oral cancer as a risk factor for second primary cancers. Cancer Lett. 220(2):185-95.

Chau CH, Clavijo CA, Deng HT, Zhang Q, Kim KJ, Qiu Y, Le AD, Ann DK. (2005). Etk/Bmx mediates the expression of stress-induced adaptive genes, VEGF, PAI-1, and iNOS via multiple signaling cascades in different cell systems. Am J Physiol Cell Physiol. 289(2):C444-54

Zhang Q, Zhang ZF, Rao JY, Sato JD, Brown J, Messadi DV, Le AD. (2004). Treatment with siRNA and antisense oligonucleotides targeted to HIF-1alpha induced apoptosis in human tongue squamous cell carcinomas. Int J Cancer. 111(6):849-57

Shetty, V. and Le AD. (2004). Oral soft tissue wound healing. Oral & Maxillofacial Surgery in Dogs and Cats. Editor: Frank J. M. Verstraete.

Aghaloo T and Le AD. (2004). Growth factors in implant site development. Oral Maxillofacial Surg Clin N Am 16:111-125.

Messadi DV, Duong HS, Zhang Q, Kelly AP, Tuan TL and Le AD. (2004). Activation of NFKappaB signal pathways in keloid fibroblasts. Arch. Derm. Research, 296(3):125-33

Mohamed KM, Le A, Duong H, Wu Y, Zhang Q, Messadi DV. (2004). Correlation between VEGF and HIF-1alpha expression in human oral squamous cell carcinoma. Exp Mol Pathol. 76(2):143-52

Wu Y, Zhang Q, Ann DK, Akhondzadeh A, Duong HS, Messadi DV, Le AD. (2004). Increased vascular endothelial growth factor may account for elevated level of plasminogen activator inhibitor-1 via activating ERK1/2 in keloid fibroblasts. Am J Physiol Cell Physiol. 286(4):C905-12

Zhang Q, Wu Y, Chau CH, Ann DK, Bertolami CN, Le AD. (2004). Crosstalk of hypoxia-mediated signaling pathways in upregulating plasminogen activator inhibitor-1 expression in keloid fibroblasts. J Cell Physiol., 199:89-97.

Le AD, Zhang Q, Wu Y, Messadi DV, Akhondzadeh A, Nguyen AL, Aghaloo TL, Kelly AP, Bertolami CN. (2004). Elevated vascular endothelial growth factor in keloids: relevance to tissue fibrosis. Cells Tissues Organs, 176(1-3): 87-94.

Chen Y, Zhao YH, Kalaslavadi TB, Hamati E, Nehrke K, Le AD, Ann DK, Wu R. (2004). Genome-wide search and identification of a novel gel-forming mucin MUC19/Muc19 in glandular tissues. Am J Respir Cell Mol Biol. 30(2):155-65.

Zhang Q, Wu Y, Ann DK, Messadi DV, Tuan TL, Kelly AP, Bertolami CN, Le AD. (2003). Mechanisms of hypoxic regulation of plasminogen activator inhibitor-1 gene expression in keloid fibroblasts. J Invest Dermatol 121:1005-1012.

Leathers R, Le AD, Black E, McQuirter JL. (2003). Orofacial injuries in underserved minority populations. Dent Clin N Am 47:127-139.

Tuan TL, Wu H, Huang EY, Chong SS, Laug W, Messadi D, Kelly P, Le A. (2003). Increased Plasminogen Activator Inhibitor-1 in keloid fibroblasts may account for their elevated collagen accumulation in fibrin gel cultures. Am J Pathol, 162(5): 1579-1589

Messadi DV, Le A, Berg S, Jewett A, Wen Z, Kelly P, Bertolami CN. (1999). Expression of apoptosis-associated genes by human dermal scar fibroblasts. Wound Rep. Reg, 7(6):511-7

Le A., Messadi DV and Bertolami CN. (1998). Expression of VEGF receptor by human dermal microvascular endothelial cells. Int J Oral Bio. 23-29.

Messadi DV, Jewett A, Berg S, Le A, Bertolami CN. (1998). Role of apoptosis in keloid formation. Int J. Oral Bio. 31-35.

Messadi DV, Le A, Berg S, Huang G, Zhuang W, Bertolami CN. (1998). Effect of TGF-beta 1 on PDGF receptors expression in human scar fibroblasts. Front Biosci. 3:a16-22

Le A, Messadi DV and Bertolami CN. (1997). Presence of CD44 on human cutaneous microvascular endothelial cells. Int. J. Oral Bio. 43-49.

Sato JD, Le AD, Kawamoto T. (1987). Derivation and assay of biological effects of monoclonal antibodies to epidermal growth factor receptors. Methods Enzymol. 146:63-81

Le A., Mendelsohn J, Sato GH et al. (1985). Receptor processing by monoclonal antibodies to epidermal growth factor receptors. Growth and Differentiation of Cells in Defined Environment (H. Murakami, D. W. Barnes, and G.H. Sato eds.), Springer-Verlag, New York, 303-306.

Gill GN, Kawamoto T, Cochet C, Le A, Sato JD, Masui H, McLeod C, Mendelsohn J. (1984). Monoclonal anti-epidermal growth factor receptor antibodies which are inhibitors of epidermal growth factor binding and antagonists of epidermal growth factor binding and antagonists of epidermal growth factor-stimulated tyrosine protein kinase activity. J. Biol. Chem. 259(12):7755-60.

Kawamoto T, Mendelsohn J, Le A, Sato GH, Lazar CS, Gill GN. (1984). Relation of epidermal growth factor receptor concentration-Growth of human epidermal carcinoma A431 cells. J Biol Chem. 259(12):7761-6

Kawamoto T, Sato JD, Le A, Polikoff J, Sato GH, Mendelsohn J. (1983). Growth stimulation of A431 cells by epidermal growth factor: identification of high-affinity receptors for epidermal growth factor by an anti-receptor monoclonal antibody. Proc Natl Acad Sci U S A. 80(5):1337-41

Kawamoto T, Sato JD, Le A, McClure DB, Sato GH. (1983). Development of a serum-free medium for growth of NS-1 mouse myeloma cells and its application to the isolation of NS-1 hybridomas. Anal Biochem. 130(2):445-53

Sato JD, Kawamoto T, Le AD, Mendelsohn J, Polikoff J, Sato GH. (1983). Biological effects in vitro of monoclonal antibodies to human epidermal growth factor receptors. Mol Biol Med. 1(5):511-29

Last Updated: 03/31/08