CCMB Faculty

CSA 106, HSC 9062
(323)442-3167 Voice
(323)442-2981 Fax
zeichner@usc.edu

Margarita Zeichner-David

Ph.D.

Research Professor

Education Experience Honors & Awards Membership Projects Publications

Education

INSTITUTION AND LOCATION	DEGREE	YEAR(s)	FIELD OF STUDY
Iberoamerican University, Mexico City	B. S.	1968	Chemistry/Biology
Center for Research & Advanced Studies, IPN, Mexico City	Ph.D.	1974	Cell Biology
NIH-NIDR, Bethesda, MD, USA	Post-Doc	1974-1977	Biochemistry

Research & Professional Experience

2001-Present	Research Professor, Division of Surgical, Therapeutic & Bioengineering Sciences, School of Dentistry, University of Southern California, Los Angeles, CA
1994-2001	Research Professor, Basic Sciences Dept., Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles, CA
1981-1994	Research Associate Professor, Basic Sciences Dept., Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles, CA
1977-Present	Faculty, Graduate Program in Craniofacial Biology, Graduate School, University of Southern California, Los Angeles, CA
1977-1981	Research Assistant Professor, Basic Sciences Dept., Laboratory for Developmental Biology, School of Dentistry, University of Southern California, Los Angeles, CA
1972-1974	Lab Instructor, Center for Research and Advanced Studies, Mexico City
1968-1970	Research Assistant, National Institute of Nutrition, Department of Biochemistry, Mexico City

Academic Honors and Awards

2001	USC Star Program Mentor of the Year 2001
1997-Present	Editorial Board, J. Intl. Oral Biol.
1997-2001	Managing Editor, Frontiers in Bioscience
1995	Honorary Member. Omicron Kappa Upsilon Dental Society. Zeta Chapter
1974-1977	Fogarty Center Fellowship
1970-1974	CONACYT fellowship
1970	Magna Cum Laude, Iberoamericana University, Mexico City

Membership

2001-2005	Member, NIDR Special Grants Review Committee
1987-1990	Ad-Hoc Reviewer, NIDCR, Special Grants Review Committee
1992-1996	Member, NIDR Special Grants Review Committee
1987-1990	Ad-Hoc Reviewer, NIH, Oral Biology and Medicine I Study Section

Research Projects

“A Molecular Insight Into Sturge-Weber's Oral Manifestations”

Principal Investigator: M. Zeichner-David.
Agency: The Sturge-Weber Foundation
Period: January 15, 2007 to January 14, 2009.
The major goal of this project is to initiate a pilot project designed to understand the oral manifestations of SWS.

"Cementogenesis: Role of Hertwig's Epithelial Root Sheath"

Principal Investigator: M. Zeichner-David.
Agency: National Institute of Dental and Craniofacial Research
Period: Feb.1, 1999 to April 30, 2008
The long-term objective of this project is to test the hypothesis that HERS cells undergo epithelial-mesenchymal transformation to become cementoblast cells which in turn deposit the cementum covering the roots.

"Tissue Engineering Using Mineral Trioxide Aggregate".

Principal Investigator: M. Zeichner-David.
Agency: American Association of Endodontist Foundation.
Period: January 1, 2006 to December 31, 2006.
The major goals of this project are to test the hypothesis that MTA can be used for tissue engineering of hard tissues damaged by disease and determine the effect of MTA on cell behavior using odontogenic cell lines in vitro and determine if MTA can stop and regenerate root and bone resorption.

"Root Resorption due to Orthodontic Movement".

Principal Investigator: M. Zeichner-David.
Agency: National Institute of Dental and Craniofacial Research
Period: May 1, 2004 to April 30, 2007.
The major goal of this project is to understand the molecular events associated with the pathological process in the rat animal model that will provide us with the starting point for future studies in humans to facilitate the treatment, prevention and possible early diagnosis of patients at risk of developing root resorption due to orthodontic treatment.

"Tissue Engineering for Periodontium Repair"

Principal Investigator: M. Zeichner-David
Agency: National Institute of Dental and Craniofacial Research
Period: July 1, 2000 to June 30, 2004
The long-term objective of this project is to test the hypothesis that HERS cells (or their products) can be used in tissue engineering to regenerate periodontal tissue.

"Characterization of Molecules Expressed as Consequence of External Root Resorption".

Principal Investigator: M. Zeichner-David.
Agency: American Association of Endodontist Foundation.
Period: July 1, 2002 to June 30, 2003.
The major goal of this project is to test the hypothesis that the application of DNA chips to mRNAs obtained from tissues subjected to pulp inflammation will result in the identification of the sequential expression of molecules that can act as signals to initiate the process of root resorpsion.

Publications

Zeichner-David M. (2006). Regeneration of periodontal tissues: cementogenesis revisited. Periodontol 2000. 41:196-217.

Zeichner-David M, Chen LS, Hsu Z, Reyna J, Caton J, Bringas P. (2006). Amelogenin and ameloblastin show growth-factor like activity in periodontal ligament cells. Eur J Oral Sci.;114 Suppl 1:244-53.

Alvarez-Perez MA, Narayanan S, Zeichner-David M, Rodriguez Carmona B, Arzate H. (2006). Molecular cloning, expression and immunolocalization of a novel human cementum-derived protein (CP-23). Bone. 38(3):409-19.

Jerome J, Brunson T, Takeoka G, Foster C, Moon HB, Grageda E, Zeichner-David M. (2005). Celebrex offers a small protection from root resorption associated with orthodontic movement. J Calif Dent Assoc. 33(12):951-9.

Plikus MV, Zeichner-David M, Mayer JA, Reyna J, Bringas P, Thewissen JGM, Snead ML, Chai Y, Chuong CM. (2005). Morphoregulation of teeth: modulating the number, size, shape and differentiation by tuning Bmp activity. Evolution & Development 7:5, 440 –457

Caton J, Bringas P Jr, Zeichner-David M. (2005). IGFs increase enamel formation by inducing expression of enamel mineralizing specific genes. Arch Oral Biol. 50(2):123-9.

Ravindranath HH, Chen L, Zeichner-David M, Ishima R, Ravindranath RMH. (2004). Interaction between the Enamel Matrix Proteins Amelogenin and Ameloblastin. Biochem Biophys Res Commun. 323,1075-1083

Vidjak FM, Zeichner-David M. (2003). Immediate-loading dental endosteal implants and the elderly patient. J Calif Dent Assoc. 31(12):917-24

Zeichner-David M, Oishi K, Su Z, Zakartchenko V, Chen LS, Arzate H, Bringas P Jr. (2003) Role of Hertwig's epithelial root sheath cells in tooth root development. Dev Dyn. 228(4):651-63.

Steele-Perkius G., Butz K. G., Lyous G. E., Zeichner-David M., Cho M. I. And Gronostajski R. M. (2003). Essential role of DFI-C/CTF transcription/replication factor in tooth roof development. Mol Cell Biol 23(3):1075-1084

Satchell PG, Anderton X, Ryu OH, Luan X, Ortega AJ, Opamen R, Berman BJ, Witherspoon DE, Gutmann JL, Yamane A, Zeichner-David M, Simmer JP, Shuler CF, Diekwisch TG. (2002). Conservation and variation in enamel protein distribution during vertebrate tooth development. J Exp Zool 294(2):91-106

Knight C, Simmons D, Gu TT, Gluhak-Heinrich J, Pavlin D, Zeichner-David M, MacDougall M. (2001). Cloning, characterization, and tissue expression pattern of mouse Nma/BAMBI during odontogenesis. J Dent Res. 80: 1895-902

Zeichner-David M (2001). Is There More To Enamel Matrix Proteins Than Biomineralization? Matrix Biol, 20: 307-316

Fincham AG, Moradian-Oldak J, Paine ML, Snead ML, Zeichner-David M (2000). Enamel Biomineralization: The Assembly and Disassembly of the Protein Extracellular Organic Matrix In: (Ferguson M., Ed.), Cambridge University Press, Cambridge, UK

Tan J, Leung W, Moradian-Oldak J, Zeichner-David M, Fincham AG (1998). Quantitative Analysis of Amelogenin Solubility. . J. Dent Res 77:1388-1396

Yamane A, Takahashi K, Mayo M, Vo H, Shum L, Zeichner-David M, Slavkin HC (1998). TGF-alpha induces the expression of myoD, myogenin and desmin during myoblast differentiation in embryonic mouse tongue development. Arch Oral Biol. 43:407-416.

Takahashi K, Yamane A, Bringas P, Caton J, Slavkin HC, Zeichner-David M (1998). Induction Of Amelogenin and Ameloblastin by Insulin and Insulin-Like Growth Factors (Igf-I And Igf-II) During Embryonic Mouse Tooth Development In Vitro. Conn Tissue Res 38: 269-278.

MacDougall M, Simmons S, Dodds A, Knight C, Luan X, Zeichner-David M, Zhang C, Ryu OH, Qian Q, Simmer JP, Hu CC (1998). Cloning, Characterization and Tissue Expression Pattern of Mouse Tuftelin cDNA. J. Dent. Res. 77:1970-1978

Tan J, Leung W, Moradian-Oldak J, Zeichner-David M, Fincham AG (1998). The pH dependent amelogenin solubility and its biological significance. Connective Tissue Research, 39: 215-221.

Zeichner-David, M, Vo H, Tan H, Diekwisch T, Berman B, Thiemann F, Alcocer MD, Hsu P, Wang T, Reyna J, Caton J, Slavkin HC, MacDougall M (1997). Timing of the expression of enamel gene products during mouse tooth development. Int. J. Dev. Biol. 41: 27-38.

Diekwisch T, Ware J, Fincham A, Zeichner-David M (1997). Immunohistochemical Similarities and differences between amelogenin and tuftelin gene expression during tooth development. J. Histochem. And Cytochem.45: 859-866

Yamane A, Takahashi K, Bringas P Jr, Amano O, Slavkin HC, Zeichner-David M (1997). Insulin-Like Growth Factor-I And II Induce an Increase in the Translational Activity of Amelogenin in the Mouse Embryonic First Molar In Vitro. Int. J. Oral Biol. 22:161-166

Moradian-Oldak J, Leung W, Simmer JP, Zeichner-David M, Fincham AG (1996). Identification of a novel proteinase "Ameloprotease I" responsible for complete degradation of amelogenin during enamel maturation. Biochem. J. 318(3):1015-1021.

Zeichner-David, M, Hall F, Williams R, Thiemann F, Yen S, MacDougall M, Slavkin HC (1995). Characterization of Protein Kinases Involved in Dentinogenesis. Connect. Tissue Res. 33:87-95.

Zeichner-David, M, Diekwisch T, Fincham AG, Lau E, MacDougall M, Moradian-Oldak J, Simmer J, Snead ML, Slavkin HC (1995). Control of ameloblast differentiation. In: Odontogenesis: Embryonic Dentition as a Tool for Developmental Biology. Intl. J. Dev. Biol. 39:69-92.

Simmer JP, Lau EC, Hu CC, Aoba T, Lacey M, Nelson D, Thiemann F, Zeichner-David M, Snead ML, Slavkin HC, Fincham AG (1994). Isolation and Characterization of a Mouse Amelogenin Expressed in Escherichia Coli. Calcif. Tissue Intl. 54: 312-319.

MacDougall M, Zeichner-David M, Crall M, Yen S, Vides J, Slavkin HC (1994). Presence of dentin phosphoprotein in molars of a patient with Dentinogenesis Imperfecta Type II. J. Craniofacial Genet. Develop. Biol. 14:26-32.

Moradian-Oldak J, Simmer JP, Sarte PE, Zeichner-David M, Fincham AG (1994). Specific cleavage of a recombinant murine amelogenin at the carboxy-terminal region by a proteinase fraction isolated from developing bovine tooth enamel. Archs. Oral Biol. 39:647-656.

Nakamura M, Bringas P, Nanci A, Zeichner-David M, Ashdown B, Slavkin HC (1994). Translocation of Enamel Proteins From Inner Enamel Epithelia to Odontoblasts During Mouse Tooth Development. Anat. Record. 238:383-396.

Carbonaro-Hall, D, Williams R, Wu L, Warburton D, Zeichner-David M, MacDougall M, Tolo V, Hall F (1993). G1 expression and multistage dynamics of cyclin A in human osteosarcoma cells. Oncogene 8:1649-1659.

MacDougall M, Rezendez R, Reyna J, Zeichner-David M (1992). Expression of Dentin Extracellular Matrix Proteins by Odontoblastic cell Cultures. In: Proceedings of the Fourth International Conference on Chemistry and Biology of Mineralized Tissues (H.C. Slavkin and P. Price, Ed.), Elsevier, Amsterdam pp. 117-124.

MacDougall M, Slavkin HC, Zeichner-David M (1992). Characteristics of Phosphorylated and Non-phosphorylated Dentin Phosphoprotein. Biochem. J. 287:651-655.

Zeichner-David, M, MacDougall M, Yen S, Hall F, Slavkin HC (1992). Protein Kinases in Dentinogenesis. Proc. Finnish Dental Society. 88:295-303.

Zeichner-David, M, MacDougall M, Davis A, Vides J, Snead ML, Slavkin HC (1989). Enamelins and amelogenins share the same amino terminal sequence. Conn. Tiss. Res. 22:123-129.

Slavkin HC, Bringas P, Bessem C, Santos V, Nakamura M, Hsu M., Snead ML, Zeichner-David M, Fincham AG (1989). Hertwig's Epithelial Root Sheath Differentiation and Initial Cementum and Bone Formation During Long-term Organ Culture of Mouse Mandibular First Molars Using serumless, Chemically-Defined Medium. J. Period. Res. 23:28-40.

Zeichner-David, M, Vides J, MacDougall M, Fincham AG, Snead ML, Bessem C, Slavkin HC (1988). Biosynthesis and characterization of rabbit enamel extracellular-matrix proteins. Biochem. J. 251:631-641.

Zeichner-David, M, MacDougall M, Vides J, Snead ML, Slavkin HC, Turkel SB, Pavlova Z (1987). Immunochemical and biochemical studies of human enamel proteins during neonatal development. J. Dent. Res. 66:50-56.

Zeichner-David, M, Vides J, Snead ML, Slavkin HC (1985). Enamel protein characterization by mRNA translation in a cell-free system. In: The Chemistry and Biology of Mineralized Tissues (W.T. Butler, Ed.), Ebsco Media, Inc., Birmingham, Alabama.

Snead ML, Lau EC, Zeichner-David M, Fincham AG, Woo SL, Slavkin HC (1985). DNA sequence for cloned cDNA for murine amelogenin reveal the amino acid sequence for enamel-specific protein. Biochem. Biophys. Res. Communs. 129:812-818.

MacDougall M, Zeichner-David M, Slavkin HC (1985). Production and characterization of antibodies against murine dentine phosphoprotein. Biochem J. 232:493-500.

Zeichner-David, M, Snead ML, Slavkin HC (1985). Genetic differences in enamel gene products amongst mammalian species. Tooth Morphogenesis and Differentiation 125:355-362.

Zeichner-David, M, MacDougall M, Vides J, Snead ML, Slavkin HC (1984). Enamel gene expression in selected vertebrates. In: Tooth Enamel IV (R.W. Fearnhead and S. Suga, Eds.) Elsevier/North Holland Publishers, Amsterdam, pp. 183-187.

Zeichner-David M, Slavkin HC, Lyaruu DM, Termine J (1983). Biosynthesis and secretion of enamel proteins during hamster tooth development. Calcif Tissue Int. 35:366-371.

Last Updated: 05/12/08