CCMB Faculty

CSA 135, HSC 9062
(323)442-1728 Voice
(323)442-2981 Fax
paine@usc.edu

Michael L. Paine

B.Sc., B.D.S., Ph.D.

Associate Professor
USC Associates Professor of Dentistry

Education Experience Honors & Awards Membership Projects Publications Book Chapters

Education

INSTITUTION AND LOCATION	DEGREE	YEAR(s)	FIELD OF STUDY
University of Southern California	Certificate	1999	Periodontology
University of Sydney, Sydney, Australia	Ph.D.	1993	Pathology
University of Sydney, Sydney, Australia	B.D.S.	1988	Dentistry
Macquarie University, Sydney, Australia	B.Sc.	1985	Biology

Research & Professional Experience

2007-present	Director, Craniofacial Biology, Graduate Program, School of Dentistry, University of Southern California, Los Angeles, CA
2006-present	Associate Professor, School of Dentistry, University of Southern California (USC), Los Angeles, CA
2004-present	USC Associates Professor of Dentistry, USC
2003-present	Medical Staff, Department of Surgery, Division of Dental Surgery, Childrens Hospital Los Angeles, California
2001-2004	Assistant Professor, School of Dentistry, USC
2000-2002	Clinical dentist part-time in the city of Whittier (California, USA). Practice limited to the diagnosis and treatment of periodontal disease and implant dentistry.
1998-2001	Research Assistant Professor, School of Dentistry, USC
1996-1998	Research Associate, School of Dentistry, USC
1996	Research Assistant Professor, Center for Craniofacial Molecular Biology, School of Dentistry, USC
1994-1996	Research Associate, Center for Craniofacial Molecular Biology, School of Dentistry, USC
1991-1992	Visiting Dental Officer, Westmead Hospital, Australia
1990-1991	Clinical dentist part-time in the city of Bondi Junction, Australia
1989	Clinical general dentist full-time in the city of Orange (Australia) including part of the year assisting with minor oral and oro-facial surgical cases at Orange Base and Dudley Private Hospitals.

Academic Honors and Awards

2006	University of Sydney Short Term Visiting Fellowship.
1999	The William J. Gies Award for the best paper published in the Journal of Dental Research in 1998 titled "Protein to protein interactions: Criteria defining the assembly of the enamel organic matrix".
1998	Outstanding Presentation Award, Ninth Intercity Periodontics Research Presentation, California Society of Periodontists.
1996-1999	University of Southern California School of Dentistry Colby Scholarship.
1995	Young Investigator Award for the Fifth International Conference on the Chemistry and Biology of Mineralized Tissue, Kohler, Wisconsin.
1993	Sydney University Faculty of Medicine Traveling Fellowship - Thomas and Ethel Mary Ewing Scholarship in Medicine.
1990-1992	National Health and Medical Research Council Dental Postgraduate Research Scholarship.

Membership

Since 1994 American Association for Dental Research.

Since 1994 American Society for Cell Biology.

Since 1995 American Academy of Periodontology.

Research Projects

"Enamel Matrix Protein Interaction "

Principal Investigator: Michael Paine, Ph.D.

Sponsor: National Institute of Dental Research

Type: R01 DE13404; Period: 3/01/2000-03/31/2011

The goal of this grant is to characterize protein-protein interactions involving the enamel extracellular matrix proteins, as well as proteins integral to the plasma membrane of ameloblast cells, and characterize how these interactions regulate enamel formation. To achieve this the molecular biochemistry of three potential membrane-bound receptors (CD63, ANXA2 and LAMP1) of the structural enamel matrix proteins, are being studied. Knowledge gained from this and related studies may lead to better dental and non-dental materials, or biomimetics. This data will be critical in the pursuit to regenerate an entire tooth, which is the ultimate goal of this and related studies.

"TIP39kDa and Tooth Formation"

Principal Investigator: Michael Paine, Ph.D.
Sponsor: National Institute of Dental and Craniofacial Research
Type: R01 DE14867; Period: 7/01/2003-06/30/2009
The goal of this grant is to establish the physiological role of TFIP11 (previously referred to as TIP39kDa) in cell biology. TFIP11 is a protein discovered in my laboratory, and at the time of discovery, the only clue to a possible function was that it contained a G-patch (glycine-rich motif) that implied a role for TFIP11 in RNA processing. Subsequent data from our group and others has identified TFIP11 as part of the spliceosome, functional complex containing approximately 200 unique proteins. Our most recent data suggests that TFIP11 plays a role in the storage of RNA splicing components, and that this storage activity is located proximal to RNA splicing events, and that these RNA splicing components can be delivered on demand.

Publications

Paine ML, Snead ML, Wang HJ, Abuladze N, Pushkin A, Liu W, Kao LY, Wall SM, Kim YH, Kurtz I. (2008). Role of NBCe1 and AE2 in secretory ameloblasts. J Dent Res. 87(4):391-5.

Zou Y, Wang H, Shapiro JL, Okamoto CT, Brookes SJ, Lyngstadaas SP, Snead ML, Paine ML. (2007) Determination of protein regions responsible for interactions of amelogenin with CD63 and LAMP1. Biochem J., 408(3):347-54.

White SN, Paine ML, Ngan AY, Miklus VG, Luo W, Wang H, Snead ML. (2007). Ectopic expression of dentin sialoprotein during amelogenesis hardens bulk enamel. J Biol Chem. 282(8):5340-5

Shapiro JL, Wen X, Okamoto CT, Wang HJ, Lyngstadaas SP, Goldberg M, Snead ML, Paine ML. (2007). Cellular uptake of amelogenin, and its localization to CD63, and Lamp1-positive vesicles. Cell Mol Life Sci. 64(2):244-56

Snead ML, Zhu DH, Lei YP, White SN, Snead CM, Luo W, Paine ML. (2006). Protein self-assembly creates a nanoscale device for biomineralization. Materials Science and Engineering: C. 26(8); 1296-1300

Bartlett JD, Ganss B, Goldberg M, Moradian-Oldak J, Paine ML, Snead ML, Wen X, White SN, Zhou YL. (2006). Protein-protein interactions of the developing enamel matrix. Curr Top Dev Biol. 74:57-115.

Zhu D, Paine ML, Luo W, Bringas P Jr, Snead ML. (2006). Altering biomineralization by protein design. J Biol Chem. 281(30):21173-82

Bartold PM, Xiao Y, Lyngstaadas SP, Paine ML, Snead ML. (2006). Principles and applications of cell delivery systems for periodontal regeneration. Periodontol 2000. 41:123-35. Review.

Swanson EC, Fong HK, Foster BL, Paine ML, Gibson CW, Snead ML, Somerman MJ. (2006). Amelogenins regulate expression of genes associated with cementoblasts in vitro. Eur J Oral Sci. 114 Suppl 1:239-43; discussion 254-6, 381-2.

Xu Y, Zhou YL, Ann DK, MacDougald OA, Shum L, Snead ML. (2006). Transcription factor sumoylation and factor YY1 serve to modulate mouse amelogenin gene expression. Eur J Oral Sci. 114 Suppl 1:169-77; discussion 201-2, 381.

Wang HJ, Tannukit S, Wen X, Shapiro JL, Snead ML, Paine ML. (2006). Using the yeast two-hybrid assay to discover protein partners for the leucine-rich amelogenin peptide and for tuftelin-interacting protein 11. Eur J Oral Sci. 114 Suppl 1:276-9.

Shapiro JL, Wang H, Wen X, Tannukit S, Paine ML. (2006). An amelogenin minigene to study alternative splicing. DNA Cell Biol. 25(1):1-5.

Paine ML, Snead ML. (2005). Tooth developmental biology: disruptions to enamel-matrix assembly and its impact on biomineralization. Orthod Craniofac Res. 8(4):239-51. Review.

White SN, Miklus VG, Chang PP, Caputo AA, Fong H, Sarikaya M, Luo W, Paine ML, Snead ML. (2005). Controlled failure mechanisms toughen the dentino-enamel junction zone. J Prosthet Dent. 94(4):330-5.

Yin Y, Huang J, Paine ML, Reinhold VN, Chasteen ND. (2005). Structural Characterization of the Major Extrapallial Fluid Protein of the Mollusc Mytilus edulis: Implications for Function. Biochemistry. 44(31):10720-31.

Paine ML, Luo W, Wang HJ, Bringas P Jr, Ngan AY, Miklus VG, Zhu DH, Macdougall M, White SN, Snead ML. (2005). Dentin sialoprotein and dentin phosphoprotein over-expression during amelogenesis. J Biol Chem. 280(36):31991-8

Wang H, Tannukit S, Zhu D, Snead ML, Paine ML. (2005). Enamel matrix protein interactions. J Bone Miner Res. 20(6):1032-40.

Wen X, Lei YP, Zhou YL, Okamoto CT, Snead ML, Paine ML. (2005). Structural organization and cellular localization of tuftelin-interacting protein 11 (TFIP11). Cell Mol Life Sci. 62(9):1038-46.

Luo W, Wen X, Wang HJ, MacDougall M, Snead ML, Paine ML. (2004). In vivo overexpression of tuftelin in the enamel organic matrix. Cells Tissues Organs. 177(4):212-20.

Shaw WJ, Campbell AA, Paine ML, Snead ML. (2004). The COOH Terminus of the Amelogenin, LRAP, Is Oriented Next to the Hydroxyapatite Surface. J Biol Chem. 279(39):40263-6

Paine ML, Zhu DH, Luo W, Snead ML. (2004). Overexpression of TRAP in the Enamel Matrix Does Not Alter the Enamel Structural Hierarchy. Cells Tissues Organs, 176:7-16.

Fong H, White SN, Paine ML, Luo W, Snead ML, Sarikaya M. (2003). Enamel structure properties controlled by engineered proteins in transgenic mice. J Bone Miner Res. 18(11):2052-9

Paine ML, Wang HJ and Snead ML. (2003). Amelogenin Self-Assembly and the Role of the Proline Located within the Carboxyl-Teleopeptide. Connective Tissue Research, 44(Suppl. 1): 52-57.

Paine ML, Luo W, Zhu DH, Bringas P Jr, Snead ML. (2003). Functional domains for amelogenin revealed by compound genetic defects. J Bone Miner Res;18(3):466-72

Paine ML, Wang HJ, Luo W, Krebsbach PH, Snead ML. (2003). A transgenic animal model resembling amelogenesis imperfecta related to ameloblastin over-expression. J Biol Chem 278:19447-52

Dunglas C, Septier D, Paine ML, Zhu DH, Snead ML, Goldberg M. (2002). Ultrastructure of Forming Enamel in Mouse Bearing a Transgene That Disrupts the Amelogenin Self-Assembly Domains. Calcif Tissue Int 71(2):155-66

Paine ML, Lei YP, Dickerson K, Snead ML. (2002). Altered amelogenin self-assembly based on mutations observed in human X-linked amelogenesis imperfecta (AIH1). J Biol Chem 2002 May 10;277(19):17112-6

White SN, Luo W, Paine ML, Fong H, Sarikaya M, Snead ML (2001). Biological organization of hydroxyapatite crystallites into a fibrous continuum toughens and controls anisotropy in human enamel. J Dent Res 2001 Jan;80(1):321-326

Paine ML, White SN, Luo W, Fong H, Sarikaya M, Snead ML (2001). Regulated gene expression dictates enamel structure and tooth function. Matrix Biol 2001 Sep;20(5-6):273-92

Moradian-Oldak J, Paine ML, Lei YP, Fincham AG, Snead ML (2000). Self-assembly properties of recombinant engineered amelogenin proteins analyzed by dynamic light scattering and atomic force microscopy. J Struct Biol 2000 Jul;131(1):27-37

Paine ML, Zhu DH, Luo W, Bringas P Jr, Goldberg M, White SN, Lei YP, Sarikaya M, Fong HK, Snead ML (2000). Enamel biomineralization defects result from alterations to amelogenin self-assembly. J Struct Biol 2000 Dec;132(3):191-200

Paine ML, Slots J, Rich SK (1998). Fluoride use in periodontal therapy: A review of the literature. Journal of the American Dental Association, 129, 69-77.

Paine ML, Krebsbach PH, Chen LS, Paine CT, Yamada Y, Deutsch D, Snead ML (1998). Protein to protein interactions: Criteria defining the assembly of the enamel organic matrix. Journal of Dental Research, 77, 496-502.

Paine CT, Paine ML, Snead ML (1998). Identification of tuftelin and amelogenin-interacting proteins using the yeast two-hybrid system. Connect Tissue Res 1998;38(1-4):257-267

Snead ML, Paine ML, Luo W, Zhu DH, Yoshida B, Lei YP, Chen LS, Paine CT, Burstein JM, Jitpukdeebudintra S, White SN, Bringas P Jr (1998). Transgene animal model for protein expression and accumulation into forming enamel. Connect Tissue Res 1998;38(1-4):279-286

Paine ML, Gibbins JR, Kefford RF (1997). Non-coexpression of collagenase and transin in malignant epithelial cells. International Journal of Oncology, 10, 615-618.

Paine ML, Snead ML (1997). Protein interactions during assembly of the organic extracellular matrix. Journal of Bone and Mineral Research, 12, 221-227.

Paine ML, Deutsch D, Snead ML (1997). Carboxyl-region of tuftelin mediates self-assembly. Connective Tissue Research, 35, 157-161.

Fincham AG, Baehner R, Chai Y, Crowe DL, Fincham C, Iskander M, Landesman HM, Lee M, Luo W, Paine M, Pereira L, Moradian-Oldak J, Rosenblum A, Snead ML, Thompson P, Wuenschell C, Zeichner-David M, Shuler CF (1997). Problem-based learning at the University of Southern California School of Dentistry. Journal of Dental Education, 61, 417-425.

Snead ML, Paine ML, Chen LS, Yoshida B, Luo W, Zhu DH, Lei YP, Liu YH, Maxson RE (1996). The murine amelogenin promoter: Developmentally regulated expression in transgenic animals. Conn. Tiss. Res. 35:1-4, pp. 41-47.

Paine ML, Gibbins JR, Choi JK, McDonald DA, Manthey AM, Walker DM, Kefford RF (1995). Intranuclear post transcriptional down-regulation responsible for loss of a keratin differentiation marker in tumour progression. Anticancer Research, 15, 2145-2154.

Whitelock JM, Paine ML, Gibbins JR, Kefford RF, O'Grady RL (1993). Multiple levels of post-transcriptional regulation of collagenase (matrix metalloproteinase 1) in an anaplastic cell line. Immunology and Cell Biology, 71, 39-47.

Paine ML, Gibbins JR, Whitelock JM, O'Grady RL, Kefford RF (1993). Unregulated and independent expression of collagenase and transin related to tumor progression. Journal of the National Cancer Institute, 85, 1425-1427.

Paine ML, Gibbins JR, Chew KE, Demetriou A, Kefford RF (1992). Loss of keratin expression in anaplastic carcinoma cells due to post-transcriptional down-regulation acting in trans. Cancer Research, 52, 6603-6611.

Book Chapters

White, S.N., Miklus, V.G., Ngan, A.Y.W., Potter, K., Luo, W., Snead, M.L. and Paine, M.L., Biomechanical function of the dentino-enamel junction reflects genetic control. Journal of the American Ceramic Society. In “Interfaces in Heterogeneous Ceramic Systems” Ceramics Transaction Series, Vol. 191, Proceedings of the 6th Pacific Rim Conferences on Ceramic and Glass Technology, (Eds. Lawrence P. Cook, Shun-ichiro Tanaka, Winnie Wong-Ng, and Robert Schwartz) John Wiley and Sons, Inc., Hoboken, New Jersey, United States of America, pp. 135-145, 2006.

Zhu, D.H., Paine, M.L., Luo, W., Laborde, J.N., Bringas, P. Jr., and Snead, M.L. Engineered amelogenin domains expressed from a genetic knock-in locus: Analysis of heterozygous female incisors. In “8th International Conference on the Chemistry and Biology of Mineralized Tissues” (Eds. William J. Landis, and Jaro Sodek) University of Toronto Press Inc., Canada, pp. 99-102, 2005.

Paine, M.L., Lei, Y.P., Luo, W., and Snead, M.L. Perturbed amelogenin protein self-assembly alters nanosphere properties resulting in defective enamel formation. In "Biological and Bioinspired Materials and Devices: Materials Research Society Symposium Proceedings" (Eds. Joanna Aizenberg, William J. Landis, Christine Orme, and Rizhi Wang), Vol. 283, Materials Research Society, Warrendale, Pennsylvania, pp. 53-62, 2004.

Paine, M.L. and Wong, J.C. Osteoblast response to pure titanium and titanium alloy. In “Bio-implant Interface: Improving Biomaterials and Tissue Reactions” (Eds. Jan Eirik Ellingsen, and S. Petter Lyngstadaas), CRC Press LLC., pp. 123-140, 2003.

Paine, M.L. Differentiation and dedifferentiation in normal and neoplastic somatic cells: A review. In “Recent Research Developments in Biological Chemistry” (Ed. S. G. Pandalai), Vol. 1, Research Signpost, Kerala, India, pp. 29-56, 2002.

Fincham AG, Moradian-Oldak J, Paine ML, Snead ML, Zeichner-David M. Enamel Biomineralization: The assembly and disassembly of an extracellular organic matrix. In Ferguson MWJ, Teaford M & Meredith-Smith (eds). Development, Function and Evolution of Teeth, Cambridge University Press, UK. 2000.

Last Updated: 05/12/08