CCMB Faculty

CSA 133, HSC 9062
(323)442-3173 Voice
(323)442-2981 Fax
wluo@usc.edu

Wen Luo

M.D.

Research Assistant Professor

Education Experience Projects Publications

Education

INSTITUTION AND LOCATION	DEGREE	YEAR(S)	FIELD OF STUDY
University of Southern California, Los Angeles, CA	Visiting Prof.	1985	Molecular Biology
Hua Nan (Zhong Shan present name) Medical College Canton, China	Post-Doc	1953-1954	Pathology
Medical College of Lingnan University, Canton, China	M.D.	1953	Medicine

Research & Professional Experience

1987-2006	Research Assistant Professor, Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles, CA
1985-1987	Visiting Scholar, Laboratory for Developmental Biology, School of Dentistry, University of Southern California, Los Angeles, CA
1984-1986	Associate Professor in Oral Pathology and Histology, Faculty of Stomatology, Jinan University Medical College, Canton, China
1979-1984	Associate Professor of Pathology, Pathology Department, Jinan University Medical College, Canton, China
1954-1979	Assistant Lecturer and Associate Professor, Department of Pathology, West China Medical University, Chengdu, Sichuan, China

Research Projects

"Determination and Expression of Amelogenin Gene Products"

Principal Investigator: Malcolm L Snead, D.D.S., Ph.D.
Agency : National Institute of Dental and Craniofacial Research
Type: R01 (DE06988, Years, 1-16) Period: July 30, 1985 to June 30, 2006
The major goals of this project are to test the hypothesis that amelogenin gene regulation is dependent upon a cascade of signals provided during instructive secondary germ layer interaction serving to pattern oral epithelium to the ameloblast phenotype.

"VonEbner Gland Gene Products"

Principal Investigator: Malcolm L. Snead, D.D.S., Ph.D.
Agency: National Institute of Dental and Craniofacial Research"
Type: R01 (DE11834, Years, 1-4) August 1, 1997 to May 31, 2001
The major goal of this project is to investigate the function of Von Ebner Gland’s products in taste transduction.

"Cell and Genetic Approaches to Enamel Biomimetics"

Principal Investigator: Malcolm L. Snead, D.D.S., Ph.D.
Agency: National Institute of Dental and Craniofacial Research
Type: R01 (DE 13045, Years, 1-5) August 1, 1998 to June 30, 2008
The major goal of this project is to determine the effects of selected tooth specific proteins on mineral deposition and composition in vitro; using transgenic animals as part of a gain function test, determine the effects of excessive amounts of tuftelin and ameloblastin proteins on tooth biomineralization in vivo.

"Training in Cellular and Molecular Biology"

Principal Investigator: Malcolm L. Snead, D.D.S., Ph.D.
Type: T32 (DE07211, Years 1-10) September 30, 1990 to June 30, 2000
The major goal of this project is to support dentist seeking advanced training in biomedical research appropriate to the mission of the NIDCR.

Publications

White SN, Paine ML, Ngan AY, Miklus VG, Luo W, Wang H, Snead ML. (2007). Ectopic expression of dentin sialoprotein during amelogenesis hardens bulk enamel. J Biol Chem. 282(8):5340-5

Snead ML, Zhu DH, Lei YP, White SN, Snead CM, Luo W, Paine ML. (2006). Protein self-assembly creates a nanoscale device for biomineralization. Materials Science and Engineering: C. 26(8); 1296-1300

Zhu D, Paine ML, Luo W, Bringas P Jr, Snead ML. (2006). Altering biomineralization by protein design. J Biol Chem. 281(30):21173-82

Xu Y, Zhou YL, Luo W, Zhu QS, Levy D, Macdougald OA, Snead ML. (2006). NF-Y and CCAAT/enhancer-binding protein alpha synergistically activate the mouse amelogenin gene. J Biol Chem. 281(23):16090-8

White SN, Miklus VG, Chang PP, Caputo AA, Fong H, Sarikaya M, Luo W, Paine ML, Snead ML. (2005). Controlled failure mechanisms toughen the dentino-enamel junction zone. J Prosthet Dent. 94(4):330-5.

Paine ML, Luo W, Wang HJ, Bringas P Jr, Ngan AY, Miklus VG, Zhu DH, Macdougall M, White SN, Snead ML. (2005). Dentin sialoprotein and dentin phosphoprotein over-expression during amelogenesis. J Biol Chem. 280(36):31991-8

Luo W, Wen X, Wang HJ, MacDougall M, Snead ML, Paine ML. (2004). In vivo overexpression of tuftelin in the enamel organic matrix. Cells Tissues Organs. 177(4):212-20.

Paine ML, Zhu DH, Luo W, Snead ML. (2004). Overexpression of TRAP in the Enamel Matrix Does Not Alter the Enamel Structural Hierarchy. Cells Tissues Organs, 176:7-16.

Fong H, White SN, Paine ML, Luo W, Snead ML, Sarikaya M. (2003). Enamel structure properties controlled by engineered proteins in transgenic mice. J Bone Miner Res. 18(11):2052-9

Paine ML, Luo W, Zhu DH, Bringas P Jr, Snead ML. (2003). Functional domains for amelogenin revealed by compound genetic defects. J Bone Miner Res;18(3):466-72

Paine ML, Wang HJ, Luo W, Krebsbach PH, Snead ML. (2003). A transgenic animal model resembling amelogenesis imperfecta related to ameloblastin over-expression. J Biol Chem 278:19447-52

White SN, Luo W, Paine ML, Fong H, Sarikaya M, Snead ML (2001). Biological organization of hydroxyapatite crystallites into a fibrous continuum toughens and controls anisotropy in human enamel. J Dent Res 2001 Jan;80(1):321-326

Paine ML, Zhu DH, Luo W, Bringas P Jr, Goldberg M, White SN, Lei YP, Sarikaya M, Fong HK, Snead ML (2000). Enamel biomineralization defects result from alterations to amelogenin self-assembly. J Struct Biol 2000 Dec;132(3):191-200

Paine CT, Paine ML, Luo W, Okamoto CT, Lyngstadaas SP, Snead ML (2000). A tuftelin-interacting protein (TIP39) localizes to the apical secretory pole of mouse ameloblasts. J. Biol. Chem. 21;275(29):22284-92.

Snead ML, Paine ML, Luo W, Zhu DH, Yoshida B, Lei YP, Paine CT, Chen LS, Burstein JM, Jitpukdeebudintra S, White SN, Bringas P Jr (1998). Transgene Animal Model for Protein Expression and Accumulation into forming Enamel. Connective Tissue Research 38: 279-286.

Jaskoll T, Luo W, Snead ML (1998). Msx-2 expression and glucocorticoid-induced overexpression in embryonic mouse submandibular glands. J. Craniofacial Genet. Dev. Biol. 18(2):79-87.

Fincham AG, Baehner R, Chai Y, Crowe DL, Fincham C, Iskandar M, Landesman HM, Lee M, Luo W, Paine M, Pereira L, Moradian-Oldak J, Roseblum A, Snead ML, Thompson P, Wuenschell C, Zeichner-David M, Shuler CF (1997). Problem-based Learning at the University of Southern California, School of Dentistry: A term report. J. Dent.Ed. 61: 417-425.

Snead ML, Paine ML, Chen LS, Yoshida B, Luo W, Zhou D, Lei YP, Liu YH, Maxson RE (1996). The murine amelogenin promoter: Developmentally regulated expression in transgenic animals. Conn. Tiss. Res. 35: 41-47.

Liu YH, Ma L, Kundu R, Ignelzi M, Sangiorgi F, Wu L, Luo W, Snead ML, Maxson R (1996). Function of the Msx2 gene in the morphogenesis of the skull. Ann NY Acad Sci. 8:785:48-58.

Liu YH, Kundu R, Wu L, Luo W, Ignelzi MA Jr, Snead ML, Maxson RE Jr (1995). Premature suture closure and ectopic cranial bone in mice expressing Msx2 transgenes in the developing skull. Dev. Bio. 92:6137-6141.

Liu YH, Ma L, Wu LY, Luo W, Kundu R, Sangiorgi F, Snead ML, Maxson R (1994). Regulation of the Msx2 homeobox gene during mouse embryogenesis: a transgene with 439 bp of 5' flanking sequence is expressed exclusively in the apical ectodermal ridge of the developing limb. Mech. In Dev. 48:187-97.

Jabs EW, Muller U, Li X, Ma L, Luo W, Haworth IS, Klisak I, Sparkes R, Warman ML, Mulliken JB, et al (1993). A mutation in the homeodomain of the human MSX2 gene in a family affected with autosomal dominant craniosynostosis. Cell 75:443-450.

Bell JR, Noveen A, Liu YH, Ma L, Dobias S, Kundo R, Luo W, Xia Y, Lusis AJ, Snead ML, Maxson R (1993). Genomic structure, chromosomal location, and evolution of the mouse HOX 8 gene. Genomics 16: 123-131.

Shum L, Sakakura Y, Bringas P, Jr, Luo W, Snead ML, Mayo M, Crohin C, Millar S, Werb Z, Buckley S (1993). EGF abrogation-induced fusilli-form dysmorphogenesis of Meckel’s cartilage during embryonic mouse mandibular morphogenesis in vitro. Development. 118(3):903-17.

Snead ML, Luo W, Hsu D, Melrose RJ, Lau EC, Stenman G (1992). Human ameloblastoma tumors express the amelogenin gene. Oral Surg. Oral Med. And Oral Path. 74:64-72.

Raaberg L, Nexo E, Buckley S, Luo W, Snead ML, Warburton D (1992). Epidermal growth factor: a novel autocrine mechanism in rat type II pheumocytes. Am. J. Respiratory Cell and Molecular. 6:44-49.

Chen LS, Couwenhoven RI, Hsu D, Luo W, Snead ML (1992). Maintenance of amelogenin gene expression by transformed epithelial cells of mouse enamel organ. Archs Oral Biol. 37:771-778.

Kidd VJ, Luo W, Xiang J, Tu F, Easton J, McCune S, Snead ML (1991). Regulated expression of a cell division control-related protein kinase during development. Cell Growth and Differentiation. 2:85-93.

Slavkin HC, Sasano Y, Kikunaga S, Bessem C, Bringas P Jr, Mayo M, Luo W, Mak G, Rall L, Snead ML (1990). Cartilage, bone and tooth induction during early embryonic mouse mandibular morphogenesis using serumless, chemically-defined medium. Conn. Tiss. Res. 24:4-51.

Luo W, Slavkin HC, Snead ML (1990). Cells from Hertwig’s epithelial root sheath do not transcribe amelogenin. J. Perio. Res. 26:42-47.

Couwenhoven R, Luo W, Rall LB, Snead ML (1990). Co-Localization of EGF transcripts and peptides by combined immunohistochemistry and in situ hybridization. J. Histochem. And Cytochem. 38:1853-1857.

Amar S, Luo W, Snead ML, Ruch JV (1989). Amelogenin gene expression in mouse incisor heterotypic recombinations. Differentiation. 41:72-77.

Snead ML, Luo W, Oliver P, Nakamura M, Don-Wheeler G, Bessem C, Bell GI, Rall LB, Slavkin HC (1989). Localization of epidermal growth factor precursor in tooth and lung during embryonic mouse development. Dev. Biol. 134(2):420-429.

Snead ML, Luo W, Rall LB, Bell GI, Slavkin HC (1988). Localization of PreProEGF messenger RNA in developing mouse molars using oligodeoxynucleotide probes. J. Dent. Res. 67:1830. Abstract.

Snead ML, Luo W, Lau EC, Slavkin HC (1988). Spatial-and temporal-restricted pattern for amelogenin gene expression during mouse molar tooth organogenesis. Development. 104:77-85.

Slavkin HC, Snead ML, Zeichner-David M, MacDougall M, Fincham AG, Lau EC, Luo W, Nakamura M, Oliver P, Evans J (1988). Factors influencing expression of dental ECM biomineralization. In: Cell and Molecular Biology of Hard Tissues (eds. G. Roden and A. Caplan), Vol. 12 (Ciba Foundation Symposium No. 135). pp. 22-41.

Luo W, Roop DR, Lau EC, Melrose RJ, Mostofi R, Snead ML (1988). In situ hybridization analysis of keratin gene expression in human ameloblastoma. J. Oral Path. 17:534-540.

Luo W, Lau EC, Mostofi R, Roop D, Slavkin HC, Snead ML (1988). Gene expression in human ameloblastoma. J. Dent. Res. (special issue) 67:265.

Luo W, Kidd V, Humphreys-Beher M, Slavkin HC, Snead ML (1987). Expression of 4 beta galactosyltransferase mRNA in ectoderm-dervied cells of the developing mouse submandibular gland. J. Cell Biology. 105:49a. Abstract.

Luo W (1985). The mechanism of wound contraction and scar contractures. J. Sci. and Med. Jinan University. 8:4.

Luo W (1984). The histochemistry and activity of hydrolytic enzymes and dehydrogenases in the developing human enamel organ. In: Tooth Enamel IV (eds. R.W. Fearnhead and S. Suga), p. 136 Amsterdam: Elsevier Sci. Pub.

Zhao YK, Huang BJ, Luo W (1983). Colour atlas of practical dermatology. (English Edition) Dragon Pearl Publication, Hong Kong, June.

Last Updated: 03/31/08