CCMB Faculty

DOH 314, HSC 9224
(323)442-6638 Voice
(323)442-6661 Fax
yhliu@usc.edu

Yi-Hsin Liu

Ph.D.

Research Assistant Professor

Education Experience Honors & Awards Projects Publications

Education

INSTITUTION AND LOCATION	DEGREE	YEAR(s)	FIELD OF STUDY
University of Southern California, Los Angeles, CA	Postdoc.		Biochem. /Mol. Biol.
University of Southern California, Los Angeles, CA	Ph. D.	1993	Microbiology
University of Califormia, Berkeley, Berkeley, CA	B.S.	1988	Genetics

Research & Professional Experience

8/2006-Present	Visiting Associate Professor of Research, Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, CA
10/2004-2007	Research Assistant Professor, Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles, CA
7/2004-10/2004	Health Scientist Administrator, Intern, Center for Scientific Review, National Institutes of Health
1996-7/2004	Research Assistant Professor, Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles, CA
1992-1996	Postdoctoral Fellow, Department of Biochemistry and Molecular Biology, School of Medicine, University of Southern California, Los Angeles, CA
1989-1992	Predoctoral Fellow, Department of Microbiology, School of Medicine, University of Southern California, Los Angeles, CA

Academic Honors and Awards

1995-1996	Knights Templar Eye Foundation Pediatric Ophthalmology Grant
1993-1996	NCI, Postdoctoral Fellowship (5T32 CAO9569)
1988-1992	NIH, Predoctoral Fellowship

Research Projects During the Last 3 Years

"Transcriptional Factor-Induced Microphthalmia"

Principal Investigator: Yi-Hsin Liu, PhD
Agency: National Eye Institute, NIH
Type: R01 EY015417; Period: Dec. 01, 2004 to Nov. 30, 2008
The major goal of this project is to advance the understanding of the underlying molecular mechanisms of eye development.

"Genes Involved in Calvarial Suture Morphogenesis"

Principal Investigator: Yi-Hsin Liu, PhD
Agency: National Institute of Dental and Craniofacial Research, NIH
Type: R29 DE12779; Period: September 30, 1998 to July 31, 2003
The major goal of this project is to obtain a complete description of patterns of expression of Smad1, and smad5 and upstream signaling components including BMP ligands and receptors in normal and abnormal development of the calvarial suture; to test the hypothesis that Smad1 and Smad5 play a essential role in the suture morphogenesis; and to establish genetic epistasis between Msx2 and Smads in the developing suture involving BMP signaling.

"Craniofacial Developmental Molecular Biology"

Principal Investigator for Project 3: Yi-Hsin Liu, PhD
Agency: National Institute of Dental and Craniofacial Research, NIH
Type: P01 DE12941; Period: August 1, 1999 to July 31,2005
The major goal of this project is to continuous long-term series of collaborations that will provide an excellent scientific environment to advance the understanding of the molecular mechanisms underlying craniofacial development.

Publications

Sun J, Zhuang FF, Mullersman JE, Chen H, Robertson EJ, Warburton D, Liu YH, Shi W. (2006). BMP4 activation and secretion are negatively regulated by an intracellular gremlin-BMP4 interaction. J Biol Chem. 281, 29349-56.

Zhuang FF and Liu YH. (2006). Usefulness of the luciferase reporter system to test the efficacy of siRNA. Methods Mol Biol. 342, 181-7.

Li M, Wu X, Zhuang F, Jiang S, Jiang M, Liu YH. (2003). Expression of murine ELL-associated factor 2 (Eaf2) is developmentally regulated. Dev Dyn. 228(2):273-80

Wu LY, Li M, Hinton DR, Guo L, Jiang S, Wang JT, Zeng A, Xie JB, Snead M, Shuler C, Maxson RE Jr, Liu YH. (2003). Microphthalmia Resulting from Msx2-Induced Apoptosis in the Optic Vesicle. Invest Ophthalmol Vis Sci 44, 2404-2412.

Qian JQ, Jiang JR, Li M, Heaphy P, Liu Y-H, Shackleford GM. (2003). Mouse Wnt9b transforming activity, tissue-specific expression and evolution. Genomics 81, 34-46.

Ma L, Liu J, Wu T, Jiang TX, Liu Y-H, Muller-Rover S, Brissette J, Peters H, Maxson R, Maas RL, Chuong C-M. (2002). 'Cyclic alopecia' in Msx2 mutants: defects in hair cycling and hair shaft differentiation. Development 130, 379-389.

Liu Y-H, Chang KJ, Qian J, Jiang M, Wu M-C, Chen D-D, Lo H-L, Hsiao C-T, Brow L, Bolen J Jr., Hu M-C, Lin W-W, Ho P-Y, Shih PY, Yao C-W, Lin W-J, Chen C-H, Wu F-Y, Lin Y-J, Xu J, Wang K. (2002). Effective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer. BMC Biotechnology 2,5 (Equal contribution).

Tan DP, Nonaka K, Nuckolls GH, Liu YH, Maxson RE, Slavkin HC, Shum L. (2002). YY1 activates Msx2 gene independent of bone morphogenetic protein signaling. Nucleic Acids Res. 30, 1213-23.

Kwang SJ, Brugger SM, Lazik A, Merrill AE, Wu LY, Liu YH, Ishii M, Sangiorgi FO, Rauchman M, Sucov HM, Maas RL, Maxson RE Jr. (2002). Msx2 is an immediate downstream effector of Pax3 in the development of the murine cardiac neural crest. Development 129:(2) 527-538

Ito Y, Sarkar P, Mi Q, Wu N, Bringas P Jr, Liu Y, Reddy S, Maxson R, Deng C, Chai Y (2001). Overexpression of Smad2 reveals its concerted action with Smad4 in regulating TGF-beta-mediated epidermal homeostasis. Dev. Biol. 236, 181-194

Sun X, Lewandoski M, Meyers EN, Liu YH, Max son RE, Martin GR (2000). Conditional inactivation of Fgf4 reveals complexity of signalling during limb bud development. Nat Genetics 25,83-86.

Liu YH, Snead ML, Maxson RE Jr (2000). Transgenic mouse models of craniofacial disorders. Methods Mol Biol 137:499-512

Liu, YH, Tang Z, Kundu RK, Wu L, Luo W, Zhu D, Sangiorgi F, Snead ML, Max son R (1999). Msx2 gene dosage influences the number of proliferative osteogenic cells in growth centers of the developing murine skull: a possible mechanism for MSX2-mediated craniosynostosis in humans. Dev Bioi. 205, 260-74.

Dodig M, Tadic T, Kronenberg MC, Dacic S, Liu, YH, Maxson RE, Rowe DW, Licthler AC (1999). Ectopic Msx2 overexpression inhibits and Msx2 antisense stimulates calvarial osteoblast differentiation. Dev BioI. 209,298-307.

Jiang TX, Liu YH, Widelitz RB, Kundy RK, Maxson RE, Chuong CM (1999). Epidermal dysplasia and abnormal hair follicles in transgenic mice overexpressing homeobox gene MSX-2. J Invest Dermatol. 113(2): 230-7

Kanzler B, Kuschert SJ, Liu YH, Mallo, M (1998). Hoxa-2 restricts the chondrogenic domain and inhibits bone formation during development of the branchial area. Development 125,2587-97.

Tan H, Ransik A, Wu H, Dobias S, Liu YH, Maxson R (1998). Disruption of primary mesenchyme cell patterning by misregulated ectodermal expression of SpMsx in sea urchin embryos. Dev Bioi. 201, 230-46.

Zemik, JH, Nowroozi N, Liu YH., Maxson R (1997). Development, maturation, and aging of the alveolar bone new insight. Dental Clinics of North America 41, 1-15.

Snead ML, Paine ML, Chen LS, Yoshida B, Luo W, Zhu DH, Lei YP, Liu YH, Maxson RE (1996). The murine amelogenin promoter: Developmentally regulated expression in transgenic animals. Conn. Tiss. Res. 35:1-4, pp. 41-47.

Liu YH., Ma L, Kundu R, Ignelzi M, Sangiorgi F, Wu L, Luo W, Snead ML, Maxson R (1996). Function of the Msx2 gene in the morphogenesis of the skull. Annals of the New York Academy of Sciences.785:48-58.

Dodig M, Kronenberg MS, Bedalov A, Kream BE, Gronowicz G, Clark SH, Mack K, Liu YH, Maxon R, Pan ZZ, Upholt WB, Rowe DW, Lichtler AC (1996). Identification of a TAAT- containing motif required for high level expression of the COL1A1 promoter in differentiated osteoblasts of transgenic mice. Journal of Biological Chemistry. 271:16422-16429.

Phippard DJ, Weber-Hall SJ, Sharpe PT, Naylor MS, Jayatalake H, Maas R, Woo I, Roberts-Clark D, Francis-West PH, Liu YH, Maxson R, Hill RE, Dale TC (1996). Regulation of Msx-1, Msx-2, Bmp-2 and Bmp-4 during foetal and postnatal mammary gland development. Development. 122:2729-2737.

Lazik R, Liu YH, Bringas P, Sangiorgi F, Maxson R (1996). A sensitive method for analysing β- galactosidase reporter gene expression in tissue sections of post implantation mouse embryos. Trends in Genetics. 12:445-447.

Ignelzi MA Jr, Liu YH, Maxson RE Jr, Snead ML (1995). Genetically engineered mice: Toolsto understand craniofacial development. Crit Rev. Oral BioI. & Med. 6:1-21. ;

Liu YH, Kundu R, Luo W, Wu LY, Ignelzi MA Jr, Snead ML, Maxson R (1995). Premature suture closure and ectopic cranial bone in mice expressing Msx2 transgenes in the developing skull. Proc. Nail. Acad. Sci. USA 92:6137-6141.

Liu YH, Ma L, Wu LY, Luo W, Kundu R, Sangiorgi F, Snead ML, Maxson R (1994). Regulation of the Msx2 homeobox gene during mouse embryogenesis: a transgene with 439 bp of 5' flanking sequence is expressed exclusively in the apical ectodermal ridge of the developing limb. Mechanisms Develop. 48:187-197.

Bell J, Noveen A, Liu YH, Ma L, Dobias S, Kundu R, Lu W, Xia Y, Lusis A, Snead ML, Maxson R (1993). Genomic structure, chromosomal location, and evolution of mouse Hox8 gene. Genomics 116:123-131.

Last Updated: 11/30/07