CCMB Staff

CSC 120N, HSC 9062
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bringas@usc.edu

Pablo Bringas, Jr.

Chief Research Specialist

Photo with President Sample 4/27/2005

Experience Publications

Research & Professional Experience

Mr.Bringas is CCMB's laboratory manager and chief research specialist. he was born and educated in Mexico and subsequently trained in experimental pathology at Walter Reed Army Medical Center in Washington D.C. He has over 38 years experience in the biomedical sciences and has been author and/or co-author of over 150 scientific papers. His primary interests are in the fields of Developmental Biology, Odontogenesis, and in vitro systems.

During the past 20 years, he has been involved in a number of science teacher training programs involving K-12 teachers from the Los Angeles Unified School District.

1983-Present	In-service training of teachers of grades K-12, Los Angeles Unified School District, Los Angeles, CA
1968-Present	Chief Research Specialist and Laboratory Manager at the Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles, CA

Publications(selected From Over 150)

Zhao H, Oka K, Bringas P, Kaartinen V, Chai Y (2008) TGF-beta type I receptor Alk5 regulates tooth initiation and mandible patterning in a type II receptor-independent manner. Developmental Biology, In Press

Oka K, Oka S, Hosokawa R, Bringas Jr. P, Brockhoff II HC, Nonaka K, Chai Y (2008) TGF-beta mediated Dlx5 signaling plays a crucial role in osteo-chondroprogenitor cell lineage determination during mandible development. Developmental Biology, In Press

Takamori K, Hosokawa R, Xu X, Deng X, Bringas P Jr, Chai Y. (2008) Epithelial fibroblast growth factor receptor 1 regulates enamel formation. J Dent Res. 87(3):238-43.

Hosokawa R, Urata M, Han J, Zehnaly A., Bringas Jr P, Nonaka K, Chai Y. (2007). TGF-beta mediated Msx2 expression controls occipital somites-derived caudal region of skull development. Dev. Biol. 310(1):140-53.

Han J, Ishii M, Bringas P Jr, Maas RL, Maxson RE Jr, Chai1 Y. (2007). Concerted action of Msx1 and Msx2 in regulating cranial neural crest cell differentiation during frontal bone development. Mechanisms of Development 124(9-10):729-45

Oka S, Oka K, Xu X, Sasaki T, Bringas P Jr, and Chai Y. (2007). Cell autonomous requirement for TGF-beta signaling during odontoblast differentiation and dentin matrix formation. Mechanism of Development 124(6):409-15.

Oka K, Oka S, Sasaki T, Ito Y, Bringas P Jr., Nonaka K, and Chai Y. (2007). The role of TGF-beta signaling in regulating chondrogenesis and osteogenesis during mandibular development. Dev. Biol. 303(1):391-404.

Zhu D, Paine ML, Luo W, Bringas P Jr, Snead ML. (2006). Altering biomineralization by protein design. J Biol Chem. 281(30):21173-82

Xu X, Han J, Ito Y, Bringas P Jr., Urata MM, Chai Y. (2006). Cell autonomous requirement for Tgfbr2 in the disappearance of medial edge epithelium during palatal fusion. Dev. Biol. 297(1):238-48.

Zeichner-David M, Chen LS, Hsu Z, Reyna J, Caton J, Bringas P. (2006). Amelogenin and ameloblastin show growth-factor like activity in periodontal ligament cells. Eur J Oral Sci.;114 Suppl 1:244-53.

Sasaki T, Ito Y, Bringas Jr. P, Chou S, Urata M, Slavkin HC, Chai Y. (2005). TGF-beta-mediated FGF signaling is critical for regulating cranial neural crest cell proliferation during frontal bone development. Development 133, 371-381

Hosokawa R, Urata MM, Ito Y, Bringas P Jr., Chai Y. (2005). Functional significance of Smad2 in regulating basal keratinocyte migration during wound healing. J Inves Dermatol. 125: 1302-1309

Zhao H, Bringas P Jr, Chai Y. (2005). An in vitro model for characterizing the post-migratory cranial neural crest cells of the first branchial arch. Dev Dyn. 235(5):1433-40

Plikus MV, Zeichner-David M, Mayer JA, Reyna J, Bringas P, Thewissen JGM, Snead ML, Chai Y, Chuong CM. (2005). Morphoregulation of teeth: modulating the number, size, shape and differentiation by tuning Bmp activity. Evolution & Development 7:5, 440 –457

Paine ML, Luo W, Wang HJ, Bringas P Jr, Ngan AY, Miklus VG, Zhu DH, Macdougall M, White SN, Snead ML. (2005). Dentin sialoprotein and dentin phosphoprotein over-expression during amelogenesis. J Biol Chem. 280(36):31991-8

Caton J, Bringas P Jr, Zeichner-David M. (2005). IGFs increase enamel formation by inducing expression of enamel mineralizing specific genes. Arch Oral Biol. 50(2):123-9.

Chen C, Chen H, Sun J, Bringas Jr P, Chen Y, Warburton D, Shi W. (2005). Smad1 expression and function during mouse embryonic lung branching morphogenesis. Am J Physiol Lung Cell Mol Physiol. 288(6):L1033-9

Cui XM, Shiomi N, Chen J, Saito T, Yamamoto T, Ito Y, Bringas Jr. P, Chai Y, Shuler CF. (2005) Overexpression of Smad2 in TGF-beta3-null mutant mice rescues cleft palate. Dev. Biol. 278, 193-202.

Saito T, Cui XM, Yamamoto T, Shiomi N, Bringas Jr. P, Shuler CF. (2005) Effect of N′-nitrosonornicotine (NNN) on murine palatal fusion in vitro. Toxicology. 207(3):475-85

Sasaki T, Ito Y, Xu X, Han J, Bringas P Jr, Maeda T, Slavkin HC, Grosschedl R, Chai Y. (2005). LEF1 is a critical epithelial survival factor during tooth morphogenesis. Dev. Biol. 278,130-143.

Xu X, Bringas P Jr, Soriano P, Chai Y. (2005). PDGFR-alpha signaling is critical for tooth cusp and palate morphogenesis. Dev. Dyn. 232(1):75-84

Zeichner-David M, Oishi K, Su Z, Zakartchenko V, Chen LS, Arzate H, Bringas P Jr. (2003) Role of Hertwig's epithelial root sheath cells in tooth root development. Dev Dyn. 2003 Dec;228(4):651-63.

Ito Y, Yeo JY, Chytil A, Han J, Bringas P Jr, Nakajima A, Shuler CF, Moses HL, Chai Y. (2003). Conditional inactivation of TGF-beta IIR in cranial neural crest causes cleft palate and calvaria defects. Development 130, 5269-5280.

Cui XM, Chai Y, Chen J, Yamamoto T, Ito Y, Bringas P, Shuler CF. (2003). TGF-beta3-dependent SMAD2 phosphorylation and inhibition of MEE proliferation during palatal fusion. Dev Dyn. 227:387-94.

Paine ML, Luo W, Zhu DH, Bringas P Jr, Snead ML. (2003). Functional domains for amelogenin revealed by compound genetic defects. J Bone Miner Res;18(3):466-72

Xu X, Jeong L, Han J, Ito Y, Bringas P Jr, Chai Y. (2003). Developmental expression of Smad1-7 suggests critical function of TGF-beta/BMP signaling in regulating epithelial-mesenchymal interaction during tooth morphogenesis. Int J Dev Biol 47(1):31-9

Zhao J, Shi W, Wang YL, Chen H, Bringas P Jr, Datto MB, Frederick JP, Wang XF, Warburton D (2002). Smad3 deficiency attenuates bleomycin-induced pulmonary fibrosis in mice. Am J Physiol Lung Cell Mol Physiol 282(3):L585-93.

Ito Y, Bringas P Jr, Mogharei A, Zhao J, Deng C, Chai Y. (2002) Receptor-regulated and inhibitory Smads in regulating TGF-beta-mediated Meckel's cartilage development. Dev.Dyn 224(1):69-78.

Ravindranath RM, Tam WY, Bringas P Jr, Santos V, Fincham AG (2001). Amelogenin-Cytokeratin 14 Interaction in Ameloblasts during Enamel Formation. J Biol Chem 276(39):36586-97

Ito Y, Sarkar P, Mi Q, Wu N, Bringas P Jr, Liu Y, Reddy S, Maxson R, Deng C, Chai Y (2001). Overexpression of Smad2 reveals its concerted action with Smad4 in regulating TGF-beta-mediated epidermal homeostasis. Dev. Biol. 236, 181-194

Paine ML, Zhu DH, Luo W, Bringas P Jr, Goldberg M, White SN, Lei YP, Sarikaya M, Fong HK, Snead ML (2000). Enamel biomineralization defects result from alterations to amelogenin self-assembly. J Struct Biol 132, 191-200.

Chai Y, Jiang X, Ito Y, Bringas P Jr, Han J, Rowitch DH, Soriano P, McMahon AP, Sucov HM (2000). Fate of the mammalian cranial neural crest during tooth and mandibular morphogenesis. Development, 127.1671-1679.

Turman JE Jr, MacDonald AS, Pawl KE, Bringas P, Chandler SH (2000). AMPA receptor subunit expression in trigeminal neurons during postnatal development. J Comp Neurol 427(1):109-23

Wen HB, Moradian-Oldak J, Leung W, Bringas P, Fincham AG (1999). Microstructures of an amelogenin gel matrix. J. Struct. Biol. 126, 42-51.

Tefft JD, Lee M, Smith S, Leinwand M, Zhao J, Bringas P Jr, Crowe DL, Warburton D (1999). Conserved function of mSpry-2, a murine homolog of Drosophila sprouty, which negatively modulates respiratory organogenesis. Curr Biol 9(4):219-22

Amano O, Bringas P, Takahashi I, Takahashi K, Yamane A, Chai Y, Nuckolls GH, Shum L, Slavkin HC (1999). Nerve Growth Factor (NGF) Induces Tooth Morphogenesis in Mouse First Branchial Arch Explants. Dev Dyn 216, 299-310.

Baehner RL, Millar-Groff S, Bringas P (1999). Developmental expression of NADPH phagocytic oxidase components in mouse embryos. Pediatr Res 46(2):152-7

Chai Y, Bringas P Jr, Mogharei A, Shuler CF, Slavkin HC. (1998). PDGF-A and PDGFR-α regulate tooth formation via autocrine mechanism during mandibular morphogenesis in vitro. Developmental Dynamics 213: 500-511

Chai Y, Bringas P Jr, Shuler C, Devaney E, Grosschedl R, Slavkin HC (1998). An early mandibular organ culture model developed to study cranial neural crest cell migration and molecular regulation of first branchial arch morphogenesis. Int. J Dev. Biol 42, 87-94.

Takahashi K, Bringas P Jr, Yamane A, Caton J, Slavkin H, Zeichner-David M (1998). Induction Of Amelogenin and Ameloblastin by Insulin and Insulin-Like Growth Factors (Igf-I And Igf-II) During Embryonic Mouse Tooth Development In Vitro. Conn Tissue Res. 38 269-278

Snead ML, Paine ML, Luo W, Zhu DH, Yoshida B, Lei YP, Chen LS, Paine CT, Burstein JM, Jitpukdeebudintra S, White SN, Bringas P Jr (1998). Transgene Animal Model for Protein Expression and Accumulation into forming Enamel. Connective Tissue Research 38(1-4), pp. 279-286.

Yamane A, Mayo M, Bringas P Jr, Chen L, Huynh M, Thai K, Shum L, Slavkin HC (1997). TGF-alpha, EGF and their cognate EGF receptor are co-expressed with desmin during embryonic, fetal and neonatal myogenesis in mouse tongue development. Develop. Dynamics 209:1-14.

Chai Y, Sasano Y, Bringas P, Mayo M, Slavkin HC, Shuler CF (1997). The characterization of the fate of midline epithelial cells during fusion of mandibular prominences in vivo. Dev. Dyn. 208. 526-535.

Lazik A, Liu Y, Bringas P, Sangiorgi F, Maxson R (1996). A sensitive method for analyzing beta-galactosidase reporter gene expression in tissue sections of mouse embryos. Trends Genet 12(11):445-7

Hoffman DS, Bringas P Jr, Slavkin HC (1995). Co-culture of contiguous developmental fields in a serumless, chemically defined medium: an in vitro model permissive or coordinate development of the mouse ear. Int.J.Dev. Biol.40: 953-964.

Fincham AG, Moradian-Oldak J, Diekwisch TG, Lyaruu DM, Wright JT, Bringas P Jr, Slavkin HC (1995). Evidence for amelogenin "nanospheres" as functional components of secretory-stage enamel matrix. J Struct Biol 115, 50-59

Nakamura M, Bringas P Jr, Nanci A, Snead ML, Zeichner-David M, Ashdown B, Slavkin HC (1994). Translocation of Enamel Proteins from Inner Enamel Epithelial to Odontoblasts during Mouse Tooth Development. The Anatomical Record. 238:383-396.

Diekwisch T, David S, Bringas P Jr, Santos V, Slavkin HC (1993). Antisense inhibition of AMEL translation demonstrates supramolecular controls for enamel HAP crystal growth during embryonic mouse mole development. Development 117:471-482.

Shum L, Sakakura Y, Bringas P Jr, Luo W, Snead ML, Mayo M, Crohin C, Millar S, Werb Z, Buckley S, Hall FL, Warburton D, Slavkin HC (1993). EGF abrogation induced fusilli-form dysmorphogenesis of Meckel's cartilage during embryonic mouse mandibular morphogenesis in vitro. Development 118:903-917.

Hu CC, Sakakura Y, Sasano Y, Shum L, Bringas P Jr, Werb Z, Slavkin HC (1992). Endogenous epidermal growth factor regulates the timing and pattern of embryonic mouse molar tooth morphogenesis. Inter. J. Develop. Biol. 36:505-516.

Lau EC, Simmer JP, Bringas P Jr, Hsu DD, Hu CC, Zeichner-David M, Thiemann F, Snead ML, Slavkin HC, Fincham AG (1992). Alternative splicing of the mouse amelogenin primary RNA transcript contributes to amelogenin heterogeneity. Biochem. Biophys. Res. Commun. Vol. 188:3, 1253-1260.

Mayo ML, Bringas P Jr, Santos V, Shum L, Slavkin HC (1992). Desmin expression during early mouse tongue morphogenesis. Int. J. Dev. Biol. 36:255-263.

Slavkin HC, Hu Y, Sakakura Y, Diekwisch T, Chai Y, Mayo M, Bringas P Jr, Simmer J, Mak G, Sasano Y, David S (1992). Gene expression, signal transduction and tissue-specific biomineralization during mammalian tooth development. Eukaryotic Gene Expression 2(4):315-329.

Nakamura M, Bringas P Jr, Slavkin HC (1991). Inner enamel epithelia synthesize and secrete enamel proteins during mouse molar occlusal "enamel-free area" development. J Craniofac Genet Dev Biol 1(2):96-104.

Slavkin HC, Kresja RJ, Fincham AG, Bringas P Jr, Santos V, Sasano Y, Snead ML, Zeichner-David M (1991). Evolution of Enamel Proteins--a Paradigm for Mechanisms of Biomineralization. (eds. S. Suga & H. Nakahama), pp. 383-389.

Kresja RJ, Bringas P Jr, Slavkin HC (1990). A Neontological Interpretation of Condont Elements Based on Agnathan Cyclostome Tooth Structure, Func. and Dev. Lethaia Vol. 23, pp. 359-378.

Kresja RJ, Bringas P Jr, Slavkin HC (1990). The Cyclostome Model: An Interpretation of Condont Element Structure and Function Based on Cyclostome Tooth Morphology, Function and Life History. Courier Forsch. Inst. Senckenberg 118:473-492, Fig. 6.

Slavkin HC, Sasano Y, Kikunaga S, Bessem C, Bringas P Jr, Mayo M, Luo W, Mak G, Rall L, Snead ML (1990). Cartilage, bone and tooth induction during early embryonic mouse mandibular morphogenesis using serumless, chemically defined medium. Connect. Tissue Res. 14:41-51.

Slavkin HC, Snead ML, Luo W, Bringas P Jr, Kikunaga S, Sasano Y, Bessem C, Mayo M, MacDougall M, Rall LB, Rappolee D. Regulatory Factors: Morphology, Biochemistry, Physiology, and Pharmacology (Proceedings of NATO ASI, Erice, Italy), (eds. A. Pecile and B. de Bernard), London: Plenum Publishing Co., Ltd., pp. 55-68.

Slavkin HC, Bringas P Jr, Sasano Y, Mayo M (1989). Early embryonic mouse mandibular morphogenesis and cytodifferentiation in serumless, chemically defined medium: a model for studies of autocrine and/or paracrine regulatory factors. J. Craniofac. Genet. & Develop. Biol. 9:185-205.

Bringas P Jr, Nakamura M, Nakamura E, Evans J, Slavkin HC (1987). Ultrastructural analysis of enamel formation during in vitro development using chemical-defined medium. Scanning Microsc 1(3):1103-8

Last Updated: 04/17/08