University of Southern California

USC Neuroscience

Takako Makita

PIBBS MENTOR

Assistant Professor

Pediatrics, Keck School of Medicine
Childrens Hospital Los Angeles

Research Topics

  1. Neurovascular interactions in growth and guidance of sympathetic axons from the superior sympathetic ganglia
  2. Autonomic (sympathetic and parasympathetic) innervations of the heart and thorax
  3. Sympathetic innervation of the kidney
  4. Development of enteric nervous system
  5. Growth and guidance of cutaneous sensory axons of the radial nerve of the forelimb
  6. Axon guidance of the cranial nerves

Research Overview

A key process in developmental neurobiology is the manner by which axons projecting from newly born neurons choose routes to reach and ultimately innervate distant target organs. This process is called axon guidance, and is characterized by the importance of instructional cues that direct axonal outgrowth. Only a limited number of guidance molecules are currently known, far less than sufficient to explain the complexity of the nervous system. One of our major projects addresses the critical role of endothelin signaling in axon guidance. Endothelins are small bioactive peptides that play important roles in numerous developmental, physiological and pathological events by interacting with specific cell surface receptors (endothelin receptors) that are members of the G-protein coupled receptor family. Although the biochemistry of endothelin signaling is well characterized in other contexts, prior to our work there was no indication that endothelins were involved in axon guidance, and therefore, little is known of how these biochemical properties are connected to the biological roles of endothelins in neural development. My lab uses mouse genetics to dissect the biological roles of endothelin signaling in neural development, and molecular and biochemistry approaches to understand the underlying mechanisms in each process. Our work has therapeutic application to axon regeneration in several congenital neurological disorders and in adult nerve injury repair, and for tissue/organ transplantations in humans.

Contact Information

E-mail:
makita@usc.edu
Mailing Address:
4650 Sunset Blvd., MS135
Los Angeles, CA 90027
Office Location:
SRI 322
Office Phone:
(323) 361-4670
Fax:
(323) 361-1549

Education

  • B.S. 1994 Chemistry - Tokyo Metropolitan University
  • Ph.D. 2004 Biochemistry and Molecular Biology - University of Southern California

Selected Publications

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Makita T,Sucov HM,Gariepy CE,Yanagisawa M,Ginty DD - Endothelins are vascular-derived axonal guidance cues for developing sympathetic neurons. - Nature [2008] Apr 10;452(7188):759-63 -PubMed

Choudhary B,Ito Y,Makita T,Sasaki T,Chai Y,Sucov HM - Cardiovascular malformations with normal smooth muscle differentiation in neural crest-specific type II TGFbeta receptor (Tgfbr2) mutant mice. - Dev Biol [2006] Jan 15;289(2):420-9 -PubMed

Makita T,Duncan SA,Sucov HM - Retinoic acid, hypoxia, and GATA factors cooperatively control the onset of fetal liver erythropoietin expression and erythropoietic differentiation. - Dev Biol [2005] Apr 1;280(1):59-72 -PubMed

Chen TH,Chang TC,Kang JO,Choudhary B,Makita T,Tran CM,Burch JB,Eid H,Sucov HM - Epicardial induction of fetal cardiomyocyte proliferation via a retinoic acid-inducible trophic factor. - Dev Biol [2002] Oct 1;250(1):198-207 -PubMed

Huang J,Powell WC,Khodavirdi AC,Wu J,Makita T,Cardiff RD,Cohen MB,Sucov HM,Roy-Burman P - Prostatic intraepithelial neoplasia in mice with conditional disruption of the retinoid X receptor alpha allele in the prostate epithelium. - Cancer Res [2002] Aug 15;62(16):4812-9 -PubMed