University of Southern California

USC Neuroscience

Daniel P Holschneider

Professor/Dept. of Psychiatry and the Behavioral Sciences, and the Depts. of Neurology, Cell & Neurobiology, and Biomedical Engineering

Research Topics

  1. Functional brain mapping of animal behaviors
  2. Behavioral and neurophysiologic characterization of animal models of neuropsychiatric disorders
  3. Technology development

Research Overview

RESEARCH INTERESTS:My laboratory's primary interest over the past 15 years has been the brain imaging of rodent behaviors as they occur in the nontethered, nonrestrained animal. A primary tool has been perfusion based mapping using the classic autoradiographic methods. Unique about our methods is that they allow us to evaluate changes in functional brain activation at the circuit level across the entire brain. Perfusion or metabolic mapping, as we have proposed, fills a gap in the current armamentarium of imaging tools in that it can deliver a 3D assessment of functional activation of the awake, nonrestrained animal, with a spatial resolution of 100 microns.
We have made significant contributions to the methodology in several areas (a) adapting statistical parametric mapping and connectivity analyses to three-dimensional autoradiographic data sets of the rat and mouse brain, (b) developing methods to allow the generation of cortical flat maps in rats and, (c) developing an implantable minipump that allows radiotracer bolus administration by remote activation in the freely moving animal. An ongoing focus is the application of functional connectivity analyses [correlation matrices of regional changes in CBF, graph theoretical analysis of network ‘hubs’ (Pajek software), effective connectivity analyses using structural equation modeling].
The lab has broad experience evaluating behavioral function in rat and mouse models of pain, brain injury, and fear, as well as exercise. Extensive experience is available for vascular surgical procedures in rats, mice and rabbits, as well as expertise in physiologic monitoring (locomotor activity, telemetric monitoring of EMG, EEG, ECG, cardiac output).

The expertise of the laboratory include functional brain mapping, animal behavior, physiologic monitoring (EEG, EMG, EKG) and basic histochemistry.

Contact Information

Web Site:
Laboratory of Vertebrate Functional Brain Mapping
E-mail:
holschne@usc.edu
Mailing Address:
Laboratory of Vertebrate Functional Brain Mapping
University of Southern California
Keck School of Medicine
Dept. of Cell and Neurobiology
1333 San Pablo St., BMT 403, MC 9112
McKibben Annex, MCA-B1
Los Angeles, CA 90089-9112
Office Location:
MCH B2
Office Phone:
(323) 442-1536
Lab Location:
MCH B1
Lab Phone:
(323)442-1585
Fax:
(323) 442-1587

Education

  • 1984 The Johns Hopkins Univ., Baltimore, MD, B.A.
  • 1988 Univ. North Carolina, Chapel Hill, NC, MD
  • 1990 Mount Zion Hospital (UCSF), San Francisco, CA, Internal Med. Internship
  • 1993 UCLA Neuropsychiatric Institute, Psychiatry Residency
  • 1994 UCLA Neuropsychiatric Institute, Geriatric Psychiatry Fellowship

Research Images

Selected Publications

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Wang Z, Myers KG, Guo Y, Ocampo MA, Jakowec MW, Holschneider DP, (2013). Functional reorganization of motor and limbic circuits after forced exercise training in a rat model of bilateral Parkinsonism, PLoS ONE, 8(11):e80058 -PubMed -Link

Holschneider DP, Guo Y, Wang Z, Roch M, Scremin OU. (2013) Remote brain networks changes after unilateral cortical impact injury and their modulation by acetylcholinesterase inhibition Journal of Neurotrauma, 30(11):907-19. -PubMed -Link

Wang Z, Ocampo MA, Pang RD, Bota M, Bradesi S, Mayer EA, Holschneider DP (2013). Alterations in Prefrontal-Limbic Functional Activation and Connectivity in Chronic Stress-Induced Visceral Hyperalgesia, PLoS ONE, 8(3):e59138 -PubMed -Link

Wang Z, Pang RD, Hernandez M, Ocampo MA, Holschneider DP (2012). Anxiolytic-like effect of pregabalin on unconditioned fear in the rat: An autoradiographic brain perfusion mapping and functional connectivity study, Neuroimage, 59(4):4168-88 -PubMed -Link

Pang RD, Klosinski L, Wang Z, Guo Y, Herman DH, Celikel T, Dong HW, Holschneider DP (2011), Mapping Functional Brain Activation in Mice Lacking the Serotonin Transporter, PLoS ONE, 2011, 6(8):e23869 -PubMed -Link

Wang Z, Guo Y, Bradesi S, Labus JS, Maarek J-M I., Lee K, Winchester WJ, Mayer EA, Holschneider DP (2009). Sex Differences In Functional Brain Activation During Noxious Visceral Stimulation In Rats, Pain, 145(1-2):120-8 -PubMed -Link

Holschneider DP., Yang J., Guo Y., Maarek J-MI. (2007) Reorganization of Functional Brain Maps After Exercise Training: Importance of Cerebellar-Thalamic-Cortical Pathway, Brain Research, 1184:96-107. -PubMed -Link

Moore WH., Holschneider DP., Givrad T., Maarek J-MI. (2006) Powering Of An Implantable Minipump By A Radiofrequency Power Link To An External Resonating Inductive Coil Driven By A Class-E Transmitter, IEEE Transactions on Biomedical Engineering, 53(8):1-3. -PubMed -Link

Holschneider DP., Yang J, Sadler TS., Nguyen PT., Givrad TK., Maarek JM. (2006) Mapping Cerebral Blood Flow Changes During Auditory Conditioned Fear in the Nontethered, Nonrestrained Rat. Neuroimage, 15;29(4):1344-58. -PubMed -Link

Nguyen PT., Holschneider DP., Maarek J-MI., Yang J., Mandelkern MA. (2004) Statistical Parametric Mapping Applied to an Autoradiographic Study of Cerebral Activation During Treadmill Walking in Rats. Neuroimage, 23:252-259. -PubMed -Link