Education:
BS Medical Technology - National Taiwan University Medical School, Taiwan
PhD 1984 Molecular Biology - Purdue University, West LaFayette, Indiana Postdoctoral Research Fellowship:
1985 Purdue University, West LaFayette, Indiana
1986 University of California, Davis Started at USC: 1994 Research Topics: Cancer Cell Biology, Signal Transduction, Cell Cycle, Growth & Proliferation, Cell Death
Research Description Our laboratory is interested in understanding the molecular mechanism(s) underlying cell proliferation and tissue-specific gene expression using epithelial and endothelial cells as a paradigm. To this end, we are employing state-of-the-art molecular approaches to elucidate the interplay among various cis-elements and trans-factors and the signal transduction pathways. Members of the protein serine/threonine and tyrosine kinases are believed to be essential components of cell signaling machinery that mediates diverse responses to extracellular stimuli in eukaryotic cells. The activation of Ras/Raf/MEK/ERK kinase cascade and/or non-receptor tyrosine kinase Etk/Bmx has been demonstrated to play an important role in initiation of cellular proliferation, differentiation and/or oncogenic transformation. Our studies have demonstrated that activators of the Ras/Raf/MEK/ERK and Etk/Bmx kinase cascades are essential for modulating gene expression in epithelial and endothelial cells. Studies are also initiated to identify the target gene(s) whose expression pattern is modulated directly or indirectly by the Ras/Raf/MEK/ERK pathway or non-receptor tyrosine kinase Etk activation. By combining molecular biological and biochemical approaches, these studies promise to provide novel insights into molecular mechanism(s) governing cell differentiation, proliferation and oncogenesis.
Selected Publications Zhou B, Ann DK, Li X, Kim KJ, Lin H, Minoo P, Crandall ED, Borok Z. - Hypertonic induction of aquaporin-5: novel role of hypoxia inducible factor-1{alpha} - Am J Physiol Cell Physiol [ 2006 ] Nov 15; . PubMed Lee YK, Thomas SN, Yang AJ, Ann DK. - Doxorubicin downregulates KAP1 SUMOylation that relieves its transcription repression on p21WAF1/CIP1 in breast cancer MCF-7 cells. - J Biol Chem [ 2006 ] Nov 1; . PubMed Xu Y, Zhou YL, Ann DK, MacDougald OA, Shum L, Snead ML. - Transcription factor sumoylation and factor YY1 serve to modulate mouse amelogenin gene expression. - Eur J Oral Sci [ 2006 ] May;114 Suppl 1:169-77; discussion 201-2, 381 . PubMed Nguyen HV, Chen JL, Zhong J, Kim KJ, Crandall ED, Borok Z, Chen Y, Ann DK. - SUMOylation attenuates sensitivity toward hypoxia- or desferroxamine-induced injury by modulating adaptive responses in salivary epithelial cells. - Am J Pathol [ 2006 ] May;168(5):1452-63 . PubMed Chung V, Zhou B, Liu X, Zhu L, Boo LM, Nguyen HV, Ann DK, Song J, Chen Y, Yen Y. - SUMOylation plays a role in gemcitabine- and bortezomib-induced cytotoxicity in human oropharyngeal carcinoma KB gemcitabine-resistant clone. - Mol Cancer Ther [ 2006 ] Mar;5(3):533-40 . PubMed Wu SK, Ann DK, Kim KJ, Lee VH. - Fine tuning of rabbit equilibrative nucleoside transporter activity by an alternatively spliced variant. - J Drug Target [ 2005 ] Sep-Nov;13(8-9):521-33 . PubMed Boo LM, Lin HH, Chung V, Zhou B, Louie SG, O'Reilly MA, Yen Y, Ann DK. - High mobility group A2 potentiates genotoxic stress in part through the modulation of basal and DNA damage-dependent phosphatidylinositol 3-kinase-related protein kinase activation. - Cancer Res [ 2005 ] Aug 1;65(15):6622-30 . PubMed Bai Y, Ann DK, Shen WC. - Recombinant granulocyte colony-stimulating factor-transferrin fusion protein as an oral myelopoietic agent. - Proc Natl Acad Sci U S A [ 2005 ] May 17;102(20):7292-6 . PubMed Chau CH, Clavijo CA, Deng HT, Zhang Q, Kim KJ, Qiu Y, Le AD, Ann DK. - Etk/Bmx mediates expression of stress-induced adaptive genes VEGF, PAI-1, and iNOS via multiple signaling cascades in different cell systems. - Am J Physiol Cell Physiol [ 2005 ] Aug;289(2):C444-54 . PubMed Chen SP, Zhou B, Willis BC, Sandoval AJ, Liebler JM, Kim KJ, Ann DK, Crandall ED, Borok Z. - Effects of transdifferentiation and EGF on claudin isoform expression in alveolar epithelial cells. - J Appl Physiol [ 2005 ] Jan;98(1):322-8 . PubMed |
