Education:
BS 1987 Microbiology - University of Florida, Gainesville, Florida
PhD 1993 Molecular Genetics & Cell Biology - University of Chicago, Chicago, Illinois
Postdoctoral Research Fellowship:
1994 - 1999 Massachusettes Institute of Technology, Cambridge, Massachusettes
Started at USC: 1999
Research Topics: Cell Cycle, Growth & Proliferation, DNA Replication, Repair, Modification
Research Description
Research in my lab focuses on three related topics:
(1) regulation of chromosomal DNA replication and the cell cycle in eukaryotic cells
(2) the role of cell cycle checkpoints in monitoring the integrity of chromosomal DNA replication and linking replication to cell cycle progression, DNA repair, and mitosis
(3) how higher order chromatin structures/modifications (e.g.: heterochromatin)influence the activity or timing or replication origin activation during S phase
We employ a combination of molecular genetic, biochemical, physiological, and genomic methods to study various aspects of these processes. A full understanding of these mechanisms will provide important insights into the regulation of gene expression and organismal development under normal circumstances and the development of cancer when these processes are defective.
10 Selected Publications:
Click here to view all the publications for this faculty
Knott SR,Viggiani CJ,Aparicio OM,Tavaré S - Strategies for analyzing highly enriched IP-chip datasets. - BMC Bioinformatics [2009] Sep 22;10():305 PubMed
Knott SR,Viggiani CJ,Aparicio OM - To promote and protect: coordinating DNA replication and transcription for genome stability. - Epigenetics [2009] Aug;4(6):362-5 PubMed
Dhillon N,Raab J,Guzzo J,Szyjka SJ,Gangadharan S,Aparicio OM,Andrews B,Kamakaka RT - DNA polymerase varepsilon, acetylases and remodellers cooperate to form a specialized chromatin structure at a tRNA insulator. - EMBO J [2009] Jul 23;(): PubMed
Viggiani CJ,Aparicio JG,Aparicio OM - ChIP-chip to analyze the binding of replication proteins to chromatin using oligonucleotide DNA microarrays. - Methods Mol Biol [2009] ;521():255-78 PubMed
Knott SR,Viggiani CJ,Tavaré S,Aparicio OM - Genome-wide replication profiles indicate an expansive role for Rpd3L in regulating replication initiation timing or efficiency, and reveal genomic loci of Rpd3 function in Saccharomyces cerevisiae. - Genes Dev [2009] May 1;23(9):1077-90 PubMed
Gasparyan HJ,Xu L,Petreaca RC,Rex AE,Small VY,Bhogal NS,Julius JA,Warsi TH,Bachant J,Aparicio OM,Nugent CI - Yeast telomere capping protein Stn1 overrides DNA replication control through the S phase checkpoint. - Proc Natl Acad Sci U S A [2009] Jan 26;(): PubMed
Szyjka SJ,Aparicio JG,Viggiani CJ,Knott S,Xu W,Tavaré S,Aparicio OM - Rad53 regulates replication fork restart after DNA damage in Saccharomyces cerevisiae. - Genes Dev [2008] Jul 15;22(14):1906-20 PubMed
Hu F,Gan Y,Aparicio OM - Identification of Clb2 residues required for Swe1 regulation of Clb2-Cdc28 in Saccharomyces cerevisiae. - Genetics [2008] Jun;179(2):863-74 PubMed
O'Neill BM,Szyjka SJ,Lis ET,Bailey AO,Yates JR 3rd,Aparicio OM,Romesberg FE - Pph3-Psy2 is a phosphatase complex required for Rad53 dephosphorylation and replication fork restart during recovery from DNA damage. - Proc Natl Acad Sci U S A [2007] May 29;104(22):9290-5 PubMed
Viggiani CJ,Aparicio OM - New vectors for simplified construction of BrdU-Incorporating strains of Saccharomyces cerevisiae. - Yeast [2006] Oct-Nov;23(14-15):1045-51 PubMed
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