Education:
BS 1987 Microbiology - University of Florida, Gainesville, Florida
PhD 1993 Molecular Genetics & Cell Biology - University of Chicago, Chicago, Illinois
Postdoctoral Research Fellowship:
1994-1999 Massachusettes Institute of Technology, Cambridge, Massachusettes
Started at USC: 1999
Research Topics: Cell Cycle, Growth & Proliferation, DNA Replication, Repair, Modification
Research Description
Research in my lab focuses on three related topics:
(1) regulation of chromosomal DNA replication and the cell cycle in eukaryotic cells
(2) the role of cell cycle checkpoints in monitoring the integrity of chromosomal DNA replication and linking replication to cell cycle progression, DNA repair, and mitosis
(3) how higher order chromatin structures/modifications (e.g.: heterochromatin)influence the activity or timing or replication origin activation during S phase
We employ a combination of molecular genetic, biochemical, physiological, and genomic methods to study various aspects of these processes. A full understanding of these mechanisms will provide important insights into the regulation of gene expression and organismal development under normal circumstances and the development of cancer when these processes are defective.
Selected Publications
Hu F, Gan Y, Aparicio OM. - Identification of Clb2 Residues Required for Swe1 Regulation of Clb2-Cdc28 in Saccharomyces cerevisiae. - Genetics [ 2008 ] Jun;179(2):863-74 . PubMed
O'Neill BM, Szyjka SJ, Lis ET, Bailey AO, Yates JR 3rd, Aparicio OM, Romesberg FE. - Pph3-Psy2 is a phosphatase complex required for Rad53 dephosphorylation and replication fork restart during recovery from DNA damage. - Proc Natl Acad Sci U S A [ 2007 ] May 29;104(22):9290-5 . PubMed
Viggiani CJ, Aparicio OM. - New vectors for simplified construction of BrdU-Incorporating strains of Saccharomyces cerevisiae. - Yeast [ 2006 ] Oct-Nov;23(14-15):1045-51 . PubMed
Xu W, Aparicio JG, Aparicio OM, Tavaré S. - Genome-wide mapping of ORC and Mcm2p binding sites on tiling arrays and identification of essential ARS consensus sequences in S. cerevisiae. - BMC Genomics [ 2006 ] Oct 26;7:276 . PubMed
Gibson DG, Bell SP, Aparicio OM. - Cell cycle execution point analysis of ORC function and characterization of the checkpoint response to ORC inactivation in Saccharomyces cerevisiae. - Genes Cells [ 2006 ] Jun;11(6):557-73 . PubMed
Dhillon N, Oki M, Szyjka SJ, Aparicio OM, Kamakaka RT. - H2A.Z functions to regulate progression through the cell cycle. - Mol Cell Biol [ 2006 ] Jan;26(2):489-501 . PubMed
Szyjka SJ, Viggiani CJ, Aparicio OM. - Mrc1 is required for normal progression of replication forks throughout chromatin in S. cerevisiae. - Mol Cell [ 2005 ] Sep 2;19(5):691-7 . PubMed
Hu F, Aparicio OM. - Swe1 regulation and transcriptional control restrict the activity of mitotic cyclins toward replication proteins in Saccharomyces cerevisiae. - Proc Natl Acad Sci U S A [ 2005 ] Jun 21;102(25):8910-5 . PubMed
Zhou XJ, Kao MC, Huang H, Wong A, Nunez-Iglesias J, Primig M, Aparicio OM, Finch CE, Morgan TE, Wong WH. - Functional annotation and network reconstruction through cross-platform integration of microarray data. - Nat Biotechnol [ 2005 ] Feb;23(2):238-43 . PubMed
Gibson DG, Aparicio JG, Hu F, Aparicio OM. - Diminished S-phase cyclin-dependent kinase function elicits vital Rad53-dependent checkpoint responses in Saccharomyces cerevisiae. - Mol Cell Biol [ 2004 ] Dec;24(23):10208-22 . PubMed
