Education:
BS 1964 Chemistry - Case Institute of Technology, Cleveland, Ohio
MS 1967 Chemistry - Univeristy of Southern California
PhD 1970 Chemistry - University of Southern California
Postdoctoral Research Fellowship:
1970 - 1973 University of Wisconsin
Started at USC: 1976 Research Topics: Cancer Cell Biology, Protein Chemistry/Enzymology, DNA & RNA
Research Description
The work in this laboratory is directed at understanding the mechanisms by which cytotoxic anti-cancer drugs interact with their intracellular targets and the molecular factors and events determining response of tumors to drugs. Innate drug sensitivity of tumors as well as acquired resistance may be due to quantitative and qualitative changes in the expression of genes of target enzymes and activating enzymes. We have developed methods for quantitating gene expression by PCR and for rapid mutation detection by RNA conformational analysis. The latter technique is being used to investigate the role of mutations in acquired drug resistance. By using quantitative PCR, we have shown substantial variation among tumors in the degree of expression of genes of drug-targeted enzymes such as thymidylate synthase. Studies are under way to identify factors that alter the normal expression of these genes in cancer cells.
10 Selected Publications:
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Kuramochi H,Hayashi K,Nakajima G,Kamikozuru H,Yamamoto M,Danenberg KD,Danenberg PV - Epidermal growth factor receptor (EGFR) mRNA levels and protein expression levels in primary colorectal cancer and corresponding liver metastases. - Cancer Chemother Pharmacol [2009] Aug 22;(): PubMed
Hoffman AC,Danenberg KD,Taubert H,Danenberg PV,Wuerl P - A three-gene signature for outcome in soft tissue sarcoma. - Clin Cancer Res [2009] Aug 15;15(16):5191-8 PubMed
Kuramochi H,Uchida K,Peters JH,Shimizu D,Vallbohmer D,Schneider S,Danenberg KD,Danenberg PV - Loss of heterozygosity at thymidylate synthase locus in Barrett's metaplasia, dysplasia, and carcinoma sequences. - BMC Cancer [2009] May 21;9(1):157 PubMed
Kobayashi H,Sugihara K,Uetake H,Higuchi T,Yasuno M,Enomoto M,Iida S,Lenz HJ,Danenberg KD,Danenberg PV - Messenger RNA expression of COX-2 and angiogenic factors in primary colorectal cancer and corresponding liver metastasis. - Int J Oncol [2009] Apr;34(4):1147-53 PubMed
Uchida K,Danenberg PV,Danenberg KD,Grem JL - Thymidylate synthase, dihydropyrimidine dehydrogenase, ERCC1, and thymidine phosphorylase gene expression in primary and metastatic gastrointestinal adenocarcinoma tissue in patients treated on a phase I trial of oxaliplatin and capecitabine. - BMC Cancer [2008] Dec 23;8():386 PubMed
Ohta S,Uemura H,Matsui Y,Ishiguro H,Fujinami K,Kondo K,Miyamoto H,Yazawa T,Danenberg K,Danenberg PV,Tohnai I,Kubota Y - Alterations of p16 and p14ARF genes and their 9p21 locus in oral squamous cell carcinoma. - Oral Surg Oral Med Oral Pathol Oral Radiol Endod [2009] Jan;107(1):81-91 PubMed
Fukui Y,Oka T,Nagayama S,Danenberg PV,Danenberg KD,Fukushima M - Thymidylate synthase, dihydropyrimidine dehydrogenase, orotate phosphoribosyltransferase mRNA and protein expression levels in solid tumors in large scale population analysis. - Int J Mol Med [2008] Dec;22(6):709-16 PubMed
Kobayashi H,Sugihara K,Uetake H,Higuchi T,Yasuno M,Enomoto M,Iida S,Azuma M,Mori R,Omori A,Lenz HJ,Danenberg KD,Danenberg PV - Messenger RNA expression of TS and ERCC1 in colorectal cancer and matched liver metastasis. - Int J Oncol [2008] Dec;33(6):1257-62 PubMed
Mori R,Xiong S,Wang Q,Tarabolous C,Shimada H,Panteris E,Danenberg KD,Danenberg PV,Pinski JK - Gene profiling and pathway analysis of neuroendocrine transdifferentiated prostate cancer cells. - Prostate [2009] Jan 1;69(1):12-23 PubMed
Oh DS,Demeester SR,Dunst CM,Mori R,Lehman BJ,Kuramochi H,Danenberg K,Danenberg P,Hagen JA,Chandrasoma P,Demeester TR - Validation of a rodent model of Barrett's esophagus using quantitative gene expression profiling. - Surg Endosc [2008] Sep 24;(): PubMed
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