Education:
BA 1986 Chemistry - Carleton College, Northfield, Minnesota
PhD 1990 Biochemistry - Duke University, Durham, North Carolina
Postdoctoral Research Fellowship:
1990 - 1993 Duke University, Durham, North Carolina
Started at USC: 1993 Research Topics: Cell Structure & Organization, Membranes & Transport, Vision Research, Drug Design, Delivery, Physiology
Research Description The major area of investigation in my laboratory is the study of the contributions of cytoskeleton and molecular motor proteins to intracellular membrane trafficking, primarily in epithelial cells. Our major project involves investigation of the contributions of these effectors to the stimulated exocytosis and compensatory endocytosis of tear proteins into ocular fluid by lacrimal acinar epithelial cells. As well, we are also interested in elucidating the molecular mechanisms underlying potential missorting of secretory products that may contribute to ocular diseases such as keratoconjunctivis sicca and Sjögren's syndrome. We are also actively investigating the contribution of motor proteins, cytoskeleton and other effectors of membrane trafficking to the intracellular transport of viruses, with projects including trafficking of adenovirus in epithelial cells and traffic of herpes simplex virus in neurons. Additional collaborative projects include studies on the membrane trafficking of transporters including PepT1 and NHE3 and the regulation of the cytoskeleton by novel signaling pathways. Finally, we actively engage in projects aimed at applying our basic findings towards the practical goals of facilitating the uptake and targeting of macromolecular drugs into target tissue.
10 Selected Publications:
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Schenke-Layland K,Xie J,Magnusson M,Angelis E,Li X,Wu K,Reinhardt DP,Maclellan WR,Hamm-Alvarez SF - Lymphocytic infiltration leads to degradation of lacrimal gland extracellular matrix structures in NOD mice exhibiting a Sjögren's syndrome-like exocrinopathy. - Exp Eye Res [2009] Oct 22;(): PubMed
Wu K,Joffre C,Li X,Macveigh-Aloni M,Hom M,Hwang J,Ding C,Gregoire S,Bretillon L,Zhong JF,Hamm-Alvarez SF - Altered expression of genes functioning in lipid homeostasis is associated with lipid deposition in NOD mouse lacrimal gland. - Exp Eye Res [2009] Apr 1;(): PubMed
Joo KI,Lei Y,Lee CL,Lo J,Xie J,Hamm-Alvarez SF,Wang P - Site-specific labeling of enveloped viruses with quantum dots for single virus tracking. - ACS Nano [2008] Aug;2(8):1553-62 PubMed
Xie J,Marchelletta RR,Thomas PB,Jacobs DT,Yarber FA,Cheney RE,Hamm-Alvarez SF,Trousdale MD - Transduced viral IL-10 is exocytosed from lacrimal acinar secretory vesicles in a myosin-dependent manner in response to carbachol. - Exp Eye Res [2008] Nov 13;(): PubMed
Marchelletta RR,Jacobs DT,Schechter JE,Cheney RE,Hamm-Alvarez SF - The class V myosin motor, myosin 5c, localizes to mature secretory vesicles and facilitates exocytosis in lacrimal acini. - Am J Physiol Cell Physiol [2008] Jul;295(1):C13-28 PubMed
Brockbank KG,MacLellan WR,Xie J,Hamm-Alvarez SF,Chen ZZ,Schenke-Layland K - Quantitative second harmonic generation imaging of cartilage damage. - Cell Tissue Bank [2008] Dec;9(4):299-307 PubMed
Yacobi NR,Demaio L,Xie J,Hamm-Alvarez SF,Borok Z,Kim KJ,Crandall ED - Polystyrene nanoparticle trafficking across alveolar epithelium. - Nanomedicine [2008] Jun;4(2):139-45 PubMed
Evans E,Zhang W,Jerdeva G,Chen CY,Chen X,Hamm-Alvarez SF,Okamoto CT - Direct interaction between Rab3D and the polymeric immunoglobulin receptor and trafficking through regulated secretory vesicles in lacrimal gland acinar cells. - Am J Physiol Cell Physiol [2008] Mar;294(3):C662-74 PubMed
Norouziyan F,Shen WC,Hamm-Alvarez SF - Tyrphostin A8 stimulates a novel trafficking pathway of apically endocytosed transferrin through Rab11-enriched compartments in Caco-2 cells. - Am J Physiol Cell Physiol [2008] Jan;294(1):C7-21 PubMed
Wang Y,Chiu CT,Nakamura T,Walker AM,Petridou B,Trousdale MD,Hamm-Alvarez SF,Mircheff AK,Schechter JE - Traffic of endogenous, transduced, and endocytosed prolactin in rabbit lacrimal acinar cells. - Exp Eye Res [2007] Dec;85(6):749-61 PubMed
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