Education:
BS 1979 Zoology - National Taiwan University, Taiwan
MA 1982 Biology - California State University, Fresno, California
PhD 1987 Genetics - University of Texas at Austin, Texas
Postdoctoral Research Fellowship:
87 - 88 88 University of Texas Health Science Center, San Antonio
88 - 90 90 Yale University, Conneticut & Stanford University, California
90 - 92 92 Stanford University School of Medicine, California
Started at USC: 1997 Research Topics: Cancer Genetics, DNA & RNA, Protein Chemistry/Enzymology, Gene Regulation/Transcription, DNA Replication, Repair, Modification, Epigenetics
Research Description DNA Methylation. The primary focus of the laboratory is to understand the mechanism and regulation of CpG methylation in mammalian cells. CpG methylation has been shown to be fundamentally important in genetic imprinting, neoplasia, and mammalian embrogenesis. We are focusing on the following areas in DNA methylation: 1) characterize transcription inhibition by methylation and chromatin structure; 2) study the effect of protein-DNA interaction on methylation pattern in human cells;3) test models for the molecular specification of genetic imprinting; 4) identify the role of specific DNA sequences and secondary structures targeting DNA demethylation and methylation; 5) study the biochemical characteristics of DNA methyltransferases. Linkage Analysis in High Risk Prostate Cancer Families. Genetic factors in prostate cancer have been suggested by several epidemiologic studies. High risk prostate cancer families from four ethnic groups have been identified and specimens from these families have been collected. We have used highly polymorphic DNA markers evenly spaced on the autosomes for linkage analysis in these families. We have identified three chromosomal regions that may harbor genes contributing to familial prostate cancer. Some candidate genes from these regions are being tested in these families.
10 Selected Publications:
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Roy D,Zhang Z,Lu Z,Hsieh CL,Lieber MR - Competition Between the RNA Transcript and the Nontemplate DNA Strand During R-Loop Formation In Vitro: A Nick Can Serve as a Strong R-loop Initiation Site. - Mol Cell Biol [2009] Oct 19;(): PubMed
Li P,Tong C,Mehrian-Shai R,Jia L,Wu N,Yan Y,Maxson RE,Schulze EN,Song H,Hsieh CL,Pera MF,Ying QL - Germline competent embryonic stem cells derived from rat blastocysts. - Cell [2008] Dec 26;135(7):1299-310 PubMed
Tsai AG,Lu H,Raghavan SC,Muschen M,Hsieh CL,Lieber MR - Human chromosomal translocations at CpG sites and a theoretical basis for their lineage and stage specificity. - Cell [2008] Dec 12;135(6):1130-42 PubMed
Wang LC,Okitsu CY,Kochounian H,Rodriguez A,Hsieh CL,Zandi E - A simple and inexpensive on-column frit fabrication method for fused-silica capillaries for increased capacity and versatility in LC-MS/MS applications. - Proteomics [2008] May;8(9):1758-61 PubMed
Huang FT,Yu K,Balter BB,Selsing E,Oruc Z,Khamlichi AA,Hsieh CL,Lieber MR - Sequence dependence of chromosomal R-loops at the immunoglobulin heavy-chain Smu class switch region. - Mol Cell Biol [2007] Aug;27(16):5921-32 PubMed
Camp NJ,Cannon-Albright LA,Farnham JM,Baffoe-Bonnie AB,George A,Powell I,Bailey-Wilson JE,Carpten JD,Giles GG,Hopper JL,Severi G,English DR,Foulkes WD,Maehle L,Moller P,Eeles R,Easton D,Badzioch MD,Whittemore AS,Oakley-Girvan I,Hsieh CL,Dimitrov L,Xu J,Stanford JL,Johanneson B,Deutsch K,McIntosh L,Ostrander EA,Wiley KE,Isaacs SD,Walsh PC,Thibodeau SN,McDonnell SK,Hebbring S,Schaid DJ,Lange EM,Cooney KA,Tammela TL,Schleutker J,Paiss T,Maier C,Grönberg H,Wiklund F,Emanuelsson M,Isaacs WB,International Consortium for Prostate Cancer Genetics - Compelling evidence for a prostate cancer gene at 22q12.3 by the International Consortium for Prostate Cancer Genetics. - Hum Mol Genet [2007] Jun 1;16(11):1271-8 PubMed
Okitsu CY,Hsieh CL - DNA methylation dictates histone H3K4 methylation. - Mol Cell Biol [2007] Apr;27(7):2746-57 PubMed
Schaid DJ,McDonnell SK,Zarfas KE,Cunningham JM,Hebbring S,Thibodeau SN,Eeles RA,Easton DF,Foulkes WD,Simard J,Giles GG,Hopper JL,Mahle L,Moller P,Badzioch M,Bishop DT,Evans C,Edwards S,Meitz J,Bullock S,Hope Q,Guy M,Hsieh CL,Halpern J,Balise RR,Oakley-Girvan I,Whittemore AS,Xu J,Dimitrov L,Chang BL,Adams TS,Turner AR,Meyers DA,Friedrichsen DM,Deutsch K,Kolb S,Janer M,Hood L,Ostrander EA,Stanford JL,Ewing CM,Gielzak M,Isaacs SD,Walsh PC,Wiley KE,Isaacs WB,Lange EM,Ho LA,Beebe-Dimmer JL,Wood DP,Cooney KA,Seminara D,Ikonen T,Baffoe-Bonnie A,Fredriksson H,Matikainen MP,Tammela TL,Bailey-Wilson J,Schleutker J,Maier C,Herkommer K,Hoegel JJ,Vogel W,Paiss T,Wiklund F,Emanuelsson M,Stenman E,Jonsson BA,Grönberg H,Camp NJ,Farnham J,Cannon-Albright LA,Catalona WJ,Suarez BK,Roehl KA - Pooled genome linkage scan of aggressive prostate cancer: results from the International Consortium for Prostate Cancer Genetics. - Hum Genet [2006] Nov;120(4):471-85 PubMed
Raghavan SC,Tsai A,Hsieh CL,Lieber MR - Analysis of non-B DNA structure at chromosomal sites in the mammalian genome. - Methods Enzymol [2006] ;409():301-16 PubMed
Higashimoto T,Panopoulos A,Hsieh CL,Zandi E - TNFalpha induces chromosomal abnormalities independent of ROS through IKK, JNK, p38 and caspase pathways. - Cytokine [2006] Apr;34(1-2):39-50 PubMed
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