Shao-Yao Ying

Research Interests

Activin-mediated inhibition of prostate cancer cell growth and its gene regulation

Activin is a growth factor that modulate the growth of several types of tumor cells, including human prostate cancer cells. We have previously shown that activin is produced and the mRNAs encoding activin and its receptors are expressed in the intact rat prostate gland as well as a human prostate cancer cell line, LNCaP. Exogenously administered recombinant human activin inhibits cell proliferation and enhances apoptosis in LNCaP cells. We hypothesized that activin/activin receptor complex may mediate expression of a series of apoptosis-related oncogenes resulting in the inhibition of cell proliferation in LNCaP cells.

To elucidate the mechanism whereby activin regulates the uncontrolled proliferation of cells in prostate cancer, we determined whether activin inhibits cell proliferation and promotes apoptosis as well as oncogenes in cell growth and prostate markers in LNCaP cells. We have developed two simple, rapid subtractive hybridization methods (USC&HSC) and isolated several up- and down- regulated genes.

Finally, we are exploring the hypothesis that oncogenes, prostate markers, growth promoting factors and androgens are involved in the activin-mediated inhibition of prostate cell growth as well as in human advanced prostate cancer. To this end, we developed techniques to generate full-length DNA libraries from less than 20 cells of tissue sections and identified the up- and down- regulated genes in these tissues.

Degrees

University of Wisconsin, PHD, 1970
University of Wisconsin, MS, 1968

MEMBERSHIPS & AFFILIATIONS

Memberships

Editorial Boards
Internal Journal of Oncology

Ying SY, Chuong CM, Lin S. (1999) Suppression of activin-induced apoptosis by novel antisense strategy in human prostate cancer cells. Biochem Biophy Res Commun 265: 669-673.

Lin SL, Chuong CM, Widelitz RB, Ying SY. (1999) In vivo analysis of cancerous gene expression by RNA-polymerase chain reaction. Nucleic Acids Res 27:4585-4589.

Ying SY, Lin S. (1999) High performance subtractive hybridization of cDNAs by covalent boding between specific complementary nucleotides. BioTechniques 26:966-979.

Ying SY, Lui HM, Lin S, Chuong CM. (1999) Generation of full-length cDNA library from single human prostate cancer cells. BioTechniques 27:410-414.

Lin S, Ying SY. (1999) Differentially expressed genes in activin-induced apoptotic LNCaP cells. Biochem Biophys Res Commun 257:187-192.

Lin SL, Chuong CM, Ying SY. (2001) A Novel mRNA-cDNA interference phenomenon for silencing bcl-2 expression in human LNCaP cells. Biochem Biophys Res Commun 281:639-644.

Lin SL, Ying SY. (2001) Generation of full-length of cDNA libraries from single cells of prostate cancer. In Lu T, Weiner M (eds.) Gene Cloning and Expression Vectors for Gene Function Analyses. pp.29-32.

Chen YG, Lui HM, Lin SL, Lee JM, Ying SY (2002) Regulation of cell proliferation, apoptosis and carcinogenesis by activin. Exptl Biol Med 227: 75-87, 2002.

Ying SY (2002) cDNA libraries: methods and protocols, a volume (volume editor: Ying SY) of the series Methods in Molecular Biology, Walker J(ed.) Humana Press, Inc.