Core Facilities:

Three Scientific and an Administrative Core are in operation. Each Core supports a technician; therefore there is cost savings for investigators by providing specialized services at the cost of supplies but not technician time. The Scientific Cores are as follows and detailed descriptions are on the following pages:

• Administrative Core (Director: Neil Kaplowitz, M.D.) Enrichment Program, P/F Projects, and Mathematical Subcore.

• Cell Culture Core (Director: Shelly Lu, M.D.) Rat and human hepatocytes, fresh isolated or plated, and various cell lines.

• Cell Biology Core (Directors: Austin Mircheff, Ph.D., Murad Ookhtens, Ph.D.) Canalicular and sinusoidal domaine-enriched liver membrane vesicles, isolated perfused liver, subcellular fractionation, confocal imaging

• Molecular Biology Core (Director: Andrew Stolz, M.D.) Oligonucleotide synthesis, isolation of Xenopus laevis oocytes for microinjection, plasmid preparation and custom services such as recombinant protein expression in bacteria. Maintenance and operational assistance with use of sophisticated equipment used in Molecular Biology (Real-time PCR, phosphoimager, luminometer, etc) as well as providing educational resources, custom expression services, development of new techniques and integration with Molecular Biology Subcores.