Primer Design »

GenBank Entrez Nucleotide Query »
Retrieve target sequence from GenBank.

Primer Selection: Image »
Select primers in a specified size and Tm range. This version generates a map of the 12 forward and 12 reverse primers with the lowest repeat scores and returns the results as aGIF image.

Primer Selection: Text »
Select primers in a specified size and Tm range. This version can generate does not a map but will list more than 12 primers in each direction. Results are returned as text.

Primer 3 »
Pick primers from a DNA sequence.

Tm Determination »
Calculate oligonucleotide melting temperatures.