Chih-Lin Hsieh , PhD

Research Interests

Familial Prostate Cancer using the linkage analysis approach.

Genome-wide scanning using 382 markers evenly spaced on the autosomes.

Candidate regions studying LOH regions in prostate cancer DNA Methylation: use of an EBV-based stable episomal system to understand the function and mechanism of DNA methylation and demethylation with elation to changes in all neoplasia.

Transcriptional regulation.
Mechanism of demethylation: study the pathway of DNA demethylation and the involvement of protein binding.

Chromatin structure.

DNA Methylation and cancer: mechanism of hypermethylation in prostate cancer

De novo DNA methylation: study of the specificity and activity of de novo methyltransferases.

Clinical Interests

Clinical cytogenetics
Molecular diagnosis

Degrees

University of Texas, Austin, PHD, 1987
California State University, MA, 1982

MEMBERSHIPS & AFFILIATIONS

Memberships

American Society of Human Genetics
American Association for Cancer Research

Selected Publications

Hsieh C-L. Evidence that Protein Binding Specifies Sites of DNA Demethylation. Mol Cell Biol 19:46-56, 1999.

Whittemore AS, Lin IG, Oakley-Girvan I, Gallagher RP, Halpern J, Kolonel LN, Wu AH and Hsieh C-L. No Evidence of Linkage for Chromosome 1q42.2-43 in Prostate Cancer. Am J Hum Genet:65:254-256, 1999.

Hsieh C-L. Evidence that Protein Binding Specifies Sites of DNA Demethylation. Mol Cell Biol 19:46-56, 1999.

Tracy RB, Hsieh C-L and Lieber MR. Inducible and Stable RNA/DNA Hybrids in the Mammalian Genome: Intermediates for Immunoglobulin Class Switch Recombination. Science 288:1058-1061, 2000.

Xu J, ?Hsieh C.-L. et al. International Consortium for Prostate Cancer Genetics (ICPCG) Combined Analysis of Hereditary Prostate Cancer Linkage to 1q24-25: Results from 772 Hereditary Prostate Cancer Families from the ICPCG. Am J Hum Genet 66:945-957, 2000.


Hsieh C-L. In Vivo Activity of De Novo Methyltransferases, Dnmt3a and Dnmt3b. Mol Cell Biol 19:8211-8218, 1999.

Karanjawala, ZE, Grawunder U, Hsieh C-L, and Lieber MR. The Nonhomologous DNA End Joining Pathway is Important for Chromosome Stability in Primary Fibroblasts. Curr Biol 9:1501-1504, 1999.

Xu, G-L, Bestor, TH, Bourc?his, D, Hsieh, C-L, Tommerup, N, Bugge M, Hulten M, Ou X., Russo J J, and Viegas-Pequignot E. Chromosome Instability and Immunodeficiency Syndrome Caused by Mutations in a DNA Methyltransferase Gene. Nature 402:187-191, 1999.


Hsieh C-L. Dynamics of Methylation Pattern. Curr Opin Genetics and Develop 10: 224-228, 2000.

Karanjawal ZE, Shi X, Hsieh C-L and Lieber MR. The Mammalian Fen-1 Locus: Structure and Conserved Sequence Features. Microb Comp Genomics 5:173-177, 2000.

Lin IG, Tomzynski TJ, Ou Q and Hsieh C-L. Modulation of DNA Binding Protein Affinity Directly Affects Target Site Demethylation. Mol Cell Biol 20:2343-2349, 2000.

Hsieh C-L, Oakley-Girvan I, Balise RR, Halpern J, Gallagher RP, Wu AH, Kolonel LN, O'Brien LE, Lin IG, Van Den Berg DJ, Teh C-Z, West DW and Whittemore AS. A Genome Screen of Families with Multiple Cases of Prostate Cancer: Evidence for Genetic Heterogeneity. Am J Hum Genet 69:148-158, 2001.

Li H, Lin, IG, and Hsieh C-L. Protein binding protects sites on stable episomes and in the chromosome from de novo methylation. Mol Cell Biol 21:3416-3424, 2001.

Lin, IG and Hsieh, C-L Chromosomal DNA demethylation specified by protein binding. EMBO Reports 2:108-112, 2001.

Chedin F, Lieber MR, and Hsieh C-L. The DNA Methyltransferase-like Protein DNMT3L Stimulates De Novo Methylation by Dnmt3a. Proc Nat Acad Sci USA 99:16916-16921, 2002.

Cui H, Onyango P, Brandenburg S, Wu Y, Hsieh C-L, and Feinberg AP. Loss of imprinting in colorectal cancer linked to hypomethylation of H19 and IGF2. Canc Res 62:6442-6446, 2002.

Karanjawala ZE, Adachi N, Irvine R, Oh EK, Shibata D, Schwarz K, Hsieh C-L and Lieber MR. The Embryonic Lethality In DNA Ligase IV-Deficient Mice Is Rescued By Deletion Of Ku: Implications for Unifying the Heterogeneous Phenotypes of NHEJ Mutants. DNA Repair 1:1017-1026, 2002.

Karanjawala ZE, Murphy N, Hinton DR, Hsieh C-L and Lieber MR Oxygen metabolism causes chromosome breaks and is associated with the neuronal apotosis observed in DNA double-strand break repair mutants. Curr Biol 12:397-402, 2002.

Irvine RA, Lin IG and Hsieh C-L. DNA Methylation Has a Local Effect on Transcription and Histone Acetylation. Mol Cell Biol 22:6689-6696, 2002.

Lin IG, Han L, Taghva A,O?Brien LE, and Hsieh C-L. Murine de novo methyltransferase, Dnmt3a, demonstrates strand asymmetry and site preference in the methylation of DNA in vitro. Mol Cell Biol 22:704-723, 2002.


Karanjawala ZE, Hsieh CL, and Lieber MR. Overexpression of Cu/Zn Superoxide Dismutase is Lethal for Mice Lacking Double-Strand Break Repair. DNA Repair, 2003.