CORE
The Center's Core facilities
provide key services to support integrated Center investigations.
ANIMAL CORE SERVICES
This core provides the rat intragastric
ethanol infusion model as well as animal models for cirrhosis which will be
used by all Center investigators. It also facilitates a training program and
animal and tissue sharing programs for Center and non-Center members.
Alcohol Models
|
Type
|
Duration
|
Chargeback
|
|
Intragastric alcohol rat
|
9~12 weeks
|
$400~$600 per
rat
|
|
Intragastric alcohol mouse
|
4~8 weeks
|
$300~$450 per
mouse
|
Liver Fibrosis Models
|
Type
|
Duration
|
Chargeback
|
|
Bile Duct Ligation
|
1~3 weeks
|
$250~$450 per rat
|
|
CCl4 and phenobarbital
|
4~8 weeks
|
$250~$600 per rat
|
|
Porcine serum
|
6~8 weeks
|
$250~$450 per rat
|
Lieber-DeCarlie Model
|
Type
|
Duration
|
Chargeback
|
|
Rat
|
4 weeks
|
$250
|
|
Rat
|
8 weeks
|
$550
|
Charge backs for research project and pilot
projects currently funded by the Center are fully waived.
Differential surcharges are placed based on
the service priority criteria.
TRAINING AND TISSUE SHARING PROGRAM
The Animal Core facilitates the training of any lab personnel from other
institutions on techniques required for setting up a rat and mouse intragastric infusion model.
It also facilitates a tissue sharing program that allows the sharing of tissue
specimens obtained from animal models produced by the Core and used by Center
investigators.
For more information, please contact:
Dr. Hide Tsukamoto
USC, MMR 414
Tel: (323) 442-5107 or (323) 442-3121
Fax: (323) 442-3126
E-Mail: htsukamo@usc.edu
NON-PARENCHYMAL LIVER CELL CORE SERVICES
1. The core will provide freshly isolated or cultured Kupffer cells (KC: hepatic macrophages), hepatic stellate cells (HSC) and sinusoidal endothelial cells (SEC) from normal rats or rat models of liver injury.
2. The priority of the services will be given to the members of the Southern California Research Center for Alcoholic Liver and Pancreatic Diseases (ALPD) and Cirrhosis.
3. The services are rendered to the qualified members on a charge back basis to recover the costs of supplies which are not supported by the center and resource grants from NIAAA.
4. The services can be rendered to non-members for higher charge backs which include personnel costs and surcharges.
Request for Services
1. A request for normal rat KC and HSC should be directed to Dr. Kinji Asahina (asahina@usc.edu) and for normal SEC to Dr. Laurie DeLeve (deleve@usc.edu) at least 3 weeks prior to the time the cells are needed.
2. A request for cell isolation services for the disease models should be made to Dr. Asahina (KC and HSC) or Dr. DeLeve (SEC) at least 2 months in advance.
Notice: We have updated the prices of KC and HSC.
Cell type |
Center Member
(Non-Member) |
Average
Yield |
Guaranteed
yield |
Rat KC |
$150 ($200)/rata |
1.8 x 107 cellsb |
9.0 x 106 cells |
Rat HSC |
$150 ($200)/rata |
5.2 x 106 cellsc |
2.6 x 106 cells |
a. These charges are based on a whole isolation procedure per rat and not on cell numbers because the cell yield varies. If KC or HSC isolated from one rat are shared, the charges will proportionally be reduced by a fraction. For example, if KC separated from one rat is equally distributed to 2 groups, the charge is $75 for each group of Center members and $100 for each group of Non-members.
b. In average, 1.8 x 107 KC are isolated from one rat following gradient ultracentrifugation and adherence on plastic dish.
c. An average yield of freshly isolated HSCs is 3.0 x 107 cells from one rat. After plating on culture dish, an average number of attached HSC is 5.2 x 106 cells per rat.
Cell type |
Center Member (Non-Member) |
Rat Endothelial cells (SEC) |
$30 ($60)/1 x 106 |
These charge backs are based on freshly isolated SEC from normal adult rats.
Differential surcharges are placed based on the service priority criteria.
MORPHOLOGY CORE SERVICES
This core provides routine and specialized morphologic
services including immunohistochemistry,
standardized, blinded histologic assessment, morphometric and image analysis.
For more information, please contact:
Dr. Samuel W. French, Morphology Core Director
Harbor-UCLA Medical Center
Tel: (310) 222-2643 | Fax: (310) 222-5333
E-Mail: sfrench@labiomed.org
ADMINISTRATIVE CORE SERVICES
This core provides the centralized administrative services to all city-wide
Center components. It also plans and implements public
relations and educational programs.
For more information, please contact:
Rosy Macias, Administrative Services Manager
USC, MMR 412
Tel: (323) 442-3109 | Fax: (323) 442-3126
E-Mail:rmacias@usc.edu
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